ADK

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Author Information

Max Kucera

Basic Information

  • ID: EG10032
  • Name: adk
  • Organism: E. coli
  • Description: This enzyme catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP and performs cellular energy homeostasis. Furthermore, it is an essential enzyme required for the biosynthesis of purine ribonucleotides while playing a vital role in regulating cell growth rates. [1] [2]
  • DNA Length: 708
  • DNA sequence:

ATG CGC ATC ATT CTT TTG GGG GCA CCT GGG GCA GGG AAG GGT ACT CAA GCA CAG TTT ATC ATG GAA AAA TAT GGC ATC CCC CAA ATC TCA ACG GGA GAT ATG TTA CGC GCC GCC GTG AAG AGC GGC TCC GAG CTG GGG AAA CAG GCC AAG GAT ATC ATG GAT GCG GGA AAG TTA GTT ACC GAC GAA CTG GTA ATC GCT TTG GTT AAA GAG CGC ATT GCT CAA GAG GAT TGT CGT AAC GGT TTC TTA TTA GAC GGC TTC CCT CGC ACC ATC CCA CAG GCT GAC GCT ATG AAA GAA GCC GGC ATT AAT GTT GAC TAT GTG TTG GAG TTT GAT GTA CCC GAC GAG TTG ATT GTA GAT CGT ATC GTC GGA CGC CGC GTG CAT GCC CCC TCT GGG CGT GTT TAT CAT GTT AAG TTT AAT CCT CCG AAA GTA GAG GGC AAG GAC GAT GTA ACG GGA GAA GAG TTA ACA ACT CGC AAG GAC GAC CAG GAG GAG ACT GTC CGC AAA CGT CTT GTC GAG TAT CAT CAA ATG ACC GCT CCG CTT ATC GGT TAT TAT AGT AAA GAG GCG GAA GCC GGT AAT ACC AAA TAC GCC AAA GTC GAT GGC ACA AAA CCA GTT GCC GAG GTG CGT GCT GAC TTG GAG AAA ATC TTA GGT TAG

  • Amino Acid length: 235
  • Amino Acid sequence:

MRIILLGAPG AGKGTQAQFI MEKYGIPQIS TGDMLRAAVK SGSELGKQAK DIMDAGKLVT DELVIALVKE RIAQEDCRNG FLLDGFPRTI PQADAMKEAG INVDYVLEFD VPDELIVDRI VGRRVHAPSG RVYHVKFNPP KVEGKDDVTG EELTTRKDDQ EETVRKRLVE YHQMTAPLIG YYSKEAEAGN TKYAKVDGTK PVAEVRADLE KILG

Function and Homologs

  • Product: Adenylate kinase
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • MULTISPECIES: adenylate kinase [Proteobacteria], 432/100%/2e-153/100%, [1]
    • Chain A, Crystal Structure Of E. Coli Adenylate Kinase Y171w Mutant, 432/100%/3e-153/99%, [2]
    • MULTISPECIES: adenylate kinase [Enterobacteriaceae], 431/100%/7e-153/99%, [3]
  • Equivalent E. coli / JCVI functional protein: Extra Credit

Expression

  • Expression Level: [high]
  • Expression Level Hypothesis: This gene is a key component in controlling the rate of growth in cells and also is essential in biosynthesis of purine ribonucleotides. These are two important processes so this gene would need to be produced a lot.
  • Expression Level References and Description: This gene was not found in the databases, but it is a very important gene due to its essential functions in controlling cell growth. [3]
  • Expression Time: [early]
  • Expression Level Hypothesis: This gene is needed to regulate the growth of cells which occurs early in the cell cycle.
  • Expression Time References and Description: Occurs during the translation period. [4]

Gene Context

  • Other Components: [fadk]
  • Possible Dependencies: needs ATP, GTP and AMP to perform functions [5]
  • Process: Translation [6]
    • Inputs: AMP + ATP
    • Outputs: 2ADP

Construct

We will handle this - not part of your assignment

  • Synthesis Score: The synthesis score: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details:
  • GenBank File: A link to the GenBank file. file
  • http://www.uniprot.org/uniprot/P69441
  • https://ecocyc.org/gene?orgid=ECOLI&id=ADENYL-KIN-MONOMER
  • https://ecocyc.org/gene?orgid=ECOLI&id=ADENYL-KIN-MONOMER
  • https://ecocyc.org/gene?orgid=ECOLI&id=EG10032#tab=GO
  • https://ecocyc.org/gene?orgid=ECOLI&id=EG10032#tab=GO
  • https://ecocyc.org/gene?orgid=ECOLI&id=EG10032#tab=GO
  • Retrieved from "http://e80boot.org/index.php?title=ADK&oldid=12016"