Blast

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  • Query cover reflects the percentage of the query that shares any similarity to the subject.
  • Identity reports the percentage of exact matching _within that coverage_.
  • Score is an absolute representation of the goodness of the alignment, calculated using an algorithm that takes into account all metrics of similarity between the sequences.
  • E-Value is a statistical result. Conceptually, you can think of this number as the probability that two sequences would have this amount of match by random chance. The lower the number, the more likely the sequences are related.