Boot order

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Work in progress

The cDNAs will be transcribed/translated in an E. coli expression system also with T7 RNA polymerase. This means there will be three different transcription systems in the tube, and two different translation systems:

At the beginning

The test tube will contain T7 RNA polymerase, the 'original' E. coli RNA polymerase, and 'original' E. coli ribosomes.

After about 1 hour

The test tube will contain T7 RNA polymerase, the 'original' E. coli RNA polymerase, 'original' E. coli ribosomes, AND whatever transcription/translation machines have been recently generated from the cDNAs.