EG10165

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Author Information

Alexander Akesson

Basic Information

  • ID: EG10165
  • Name: glucose-specific phosphotransferase enzyme IIA component, crr
  • Organism: E. coli
  • Description: EG10165 codes for the glucose-specific phosphotransferase enzyme IIA component which is part of the sugar transporting phosphotransferase system (PTS) [1]. PTS transports sugars such as glucose, mannose, etc. into prokaryotic cells and then phosphorylates them [2]. In this case, the glucose-specific phosphotransferase enzyme IIA component, is the enzyme that catalyzes the reaction transporting glucose into the cell. It catalyzes the following reaction: [Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) <=> [protein]-L-histidine + D-glucose 6-phosphate [3].
  • DNA Length: 510 base pairs.
  • DNA sequence:

ATG GGG TTG TTC GAC AAA TTG AAG TCC TTG GTA TCA GAC GAC AAA AAG GAT ACG GGA ACA ATC GAG ATT ATT GCT CCG TTA AGT GGG GAG ATT GTA AAC ATC GAA GAC GTG CCG GAC GTT GTA TTT GCC GAA AAG ATC GTT GGC GAC GGT ATT GCC ATT AAG CCT ACG GGT AAC AAG ATG GTA GCT CCC GTT GAT GGG ACC ATC GGG AAA ATT TTT GAG ACC AAT CAC GCA TTT TCT ATT GAG TCG GAC AGT GGA GTG GAG TTA TTC GTC CAT TTC GGC ATC GAC ACA GTT GAG CTG AAG GGA GAG GGT TTT AAG CGT ATC GCT GAG GAG GGG CAG CGT GTA AAA GTC GGC GAT ACA GTG ATT GAG TTT GAT CTT CCC CTG CTG GAA GAG AAG GCA AAA AGC ACG CTG ACA CCT GTA GTC ATT TCT AAT ATG GAC GAA ATC AAA GAG CTG ATC AAA CTG TCC GGT TCT GTT ACG GTT GGC GAG ACT CCA GTA ATT CGC ATC AAG AAG TAA

  • Amino Acid length: 169 amino acids.
  • Amino Acid sequence:

MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGEGFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVGETPVIRIKK

Function and Homologs

  • Product: glucose-specific phosphotransferase enzyme IIA component
  • Module: Phosphotransferase system (phosphoenolpyruvate-protein phosphotransferase is part of a larger system, PTS, which transports sugars into the cell).
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • glucose-specific phosphotransferase enzyme IIA component [Shigella sp. SF-2015], Max score: 324, Query Cover: 100%, E-Value: 3e-112, Ident: 99%, WP_000522243.1
    • PTS glucose transporter subunit IIA [Escherichia albertii], Max score: 324, Query Cover: 100%, E-Value: 7e-112, Ident: 99%, WP_059279272.1
    • PTS glucose transporter subunit IIA [Shigella dysenteriae], Max score: 323, Query Cover: 100%, E-Value: 1e-111, Ident: 90%, WP_000522248.1
  • Equivalent E. coli / JCVI functional protein: A link to the identified E. coli functional analog of the protein encoded by the gene, or a link back to the JCVI gene matching this E. coli version. For example, MMSYN1 0234.

Expression

  • Expression Level: high
  • Expression Level Hypothesis: The crr gene is part of the the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). It is needed by the cell since it is the enzyme that catalyzes the reaction transporting glucose into the cell. Glucose is a vital component for the cell.[4].
  • Expression Level References and Description: The ptsP gene was not available in the Mycoplasma genitalium file, but it was found in the E. coli proteome.
  • Expression Time: At which time should the gene be expressed in the lifecycle of our organism? Early
  • Expression Level Hypothesis: Glucose is needed for cell respiration, which is essential for cellular growth. Thus, the gene needs to be expressed early.
  • Expression Time References and Description: Research description above and UniProt.

Gene Context

  • Other Components: glpK glpK, glycerol kinase
  • Possible Dependencies: Histidine kinase A histidine kinase is needed since it is the enzyme that catalyzes the reaction that adds a phosphate group to the protein L-histidine [1]. This in turn, creates [Protein]-N(pi)-phospho-L-histidine which is needed for this reaction.[2][3].
  • Process: phosphoenolpyruvate-dependent sugar phosphotransferase system
    • Inputs: [Protein]-N(pi)-phospho-L-histidine, D-glucose(Side 1)
    • Outputs: [protein]-L-histidine, D-glucose 6-phosphate(Side 2)
    • Reference: [4]

Construct

  • Synthesis Score: The synthesis score of your construct: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details: For example, had to use a rare codon to fix folding energy;
  • GenBank File: A link to the GenBank file. file
  • http://enzyme.expasy.org/EC/2.7.1.199]
  • https://en.wikipedia.org/wiki/Phosphotransferase#Phosphotransferase_system
  • http://enzyme.expasy.org/EC/2.7.1.199]
  • http://www.uniprot.org/uniprot/P69783
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