Michael Morrissey: 06024019
- ID: EG10407
- Name: gltX
- Organism: E. coli
- UniProt ID: P04805
- Description: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). Phosphorylation of GltX by HipA prevents it from being charged, leading to an increase in uncharged tRNA(Glu). This induces amino acid starvation and the stringent response via RelA/SpoT and increased ppGpp levels, which inhibits replication, transcription, translation and cell wall synthesis, reducing growth and leading to multidrug resistance and persistence. Source: uniprot.org
- DNA Length: 1,416 base pairs.
- DNA sequence:
atgAAAATCA AAACTCGCTT CGCGCCAAGC CCAACAGGCT ATCTGCACGT TGGCGGCGCG
CGTACTGCTC TTTACTCCTG GCTTTTTGCA CGTAACCACG GCGGTGAGTT CGTGCTGCGT
ATTGAAGACA CCGATCTTGA GCGTTCCACG CCGGAAGCTA TCGAAGCCAT TATGGATGGC
ATGAACTGGC TGAGCCTGGA GTGGGATGAA GGTCCGTACT ACCAGACCAA ACGTTTTGAT
CGCTACAACG CGGTGATCGA TCAGATGCTG GAAGAGGGCA CTGCTTATAA ATGCTATTGC
TCTAAAGAGC GCCTGGAAGC GCTGCGCGAA GAGCAAATGG CGAAAGGTGA GAAGCCGCGT
TATGACGGTC GCTGCCGCCA CAGCCATGAG CATCATGCTG ATGATGAACC GTGTGTTGTA
CGTTTTGCTA ACCCGCAGGA AGGTTCTGTT GTTTTTGACG ATCAGATCCG TGGTCCGATC
GAGTTCAGCA ACCAGGAACT GGACGATCTT ATTATCCGCC GTACCGATGG TTCCCCAACC
TATAACTTCT GTGTGGTTGT CGATGACTGG GATATGGAAA TCACCCACGT TATCCGTGGC
GAAGACCATA TCAACAACAC GCCACGCCAG ATCAACATTC TTAAGGCCCT GAAAGCGCCG
GTGCCGGTTT ACGCGCACGT TTCTATGATC AATGGCGATG ACGGTAAAAA ACTGTCCAAA
CGTCACGGGG CAGTCAGCGT AATGCAGTAT CGTGATGACG GTTATTTGCC AGAAGCACTG
CTGAACTATC TGGTGCGTCT GGGCTGGTCC CACGGCGATC AGGAAATCTT CACTCGTGAA
GAGATGATCA AATACTTCAC TTTGAATGCC GTCAGCAAAT CTGCCAGTGC GTTCAACACC
GACAAGCTGC TGTGGCTGAA CCATCACTAC ATTAACGCGC TGCCGCCGGA GTATGTTGCT
ACTCACTTAC AGTGGCACAT TGAGCAGGAA AATATCGATA CCCGTAACGG CCCGCAGCTG
GCTGATCTGG TGAAACTGCT GGGCGAACGC TGCAAGACGC TGAAAGAGAT GGCACAGAGC
TGCCGTTATT TCTACGAAGA TTTTGCTGAG TTCGATGCCG ACGCCGCGAA AAAACATCTG
CGTCCGGTAG CGCGTCAGCC GCTGGAAGTG GTTCGTGACA AACTGGCCGC GATTACTGAC
TGGACCGCTG AAAACGTTCA TCACGCTATT CAGGCGACGG CGGATGAGCT GGAAGTGGGT
ATGGGTAAAG TTGGTATGCC GCTGCGTGTC GCCGTAACCG GTGCGGGGCA GTCTCCAGCA
CTGGATGTTA CCGTTCACGC AATTGGTAAG ACCCGCAGTA TCGAGCGTAT CAACAAAGCG
CTGGATTTTA TTGCTGAACG CGAAAATCAG CAGtaa
- Amino Acid length: 471 amino acids.
- Amino Acid sequence:
MKIKTRFAPS PTGYLHVGGA RTALYSWLFA RNHGGEFVLR IEDTDLERST PEAIEAIMDG
MNWLSLEWDE GPYYQTKRFD RYNAVIDQML EEGTAYKCYC SKERLEALRE EQMAKGEKPR
YDGRCRHSHE HHADDEPCVV RFANPQEGSV VFDDQIRGPI EFSNQELDDL IIRRTDGSPT
YNFCVVVDDW DMEITHVIRG EDHINNTPRQ INILKALKAP VPVYAHVSMI NGDDGKKLSK
RHGAVSVMQY RDDGYLPEAL LNYLVRLGWS HGDQEIFTRE EMIKYFTLNA VSKSASAFNT
DKLLWLNHHY INALPPEYVA THLQWHIEQE NIDTRNGPQL ADLVKLLGER CKTLKEMAQS
CRYFYEDFAE FDADAAKKHL RPVARQPLEV VRDKLAAITD WTAENVHHAI QATADELEVG
MGKVGMPLRV AVTGAGQSPA LDVTVHAIGK TRSIERINKA LDFIAERENQ Q
Function and Homologs
- Functional Category: Translation.
- Product: glutamate—tRNA ligase.
- Module:tRNA loading.
- Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
- Equivalent MMSYN1_0126.
- Expression Level: High
- Expression Level Hypothesis: E. Coli may have high levels of proteins that involve glutamate as one or more of their Amino Acids. If this is the case, it makes sense that the cell would need several tRNA binding agents for glutamate to aid in the synthesis of these proteins.
- Expression Level References and Description: Expression Level was found in the following documentation: Proteomic Expression Data
- Expression Time: right at the beginning
- Expression Level Hypothesis: Because this protein is integral in transcribing future proteins that use the amino acid glutamate, it is essential to have right at the begginning to accommodate any other early proteins that require glutamate.
- Expression Time References and Description: Expression time was estimated based on information on gene function found at 
- Other Components: HipA, this is an inhibitor that acts in this functional module as an inhibitor, controlling production levels
- Possible Dependencies: Zn2+, ATP, glutamate, tRNA(Glu)
- Process: attaches glutamate to the appropriate tRNA
- Inputs: ATP, L-glutamate, tRNA(Glu)
- Outputs: AMP, diphosphate, L-glutamyl-tRNA(Glu)
We will handle this - not part of your assignment
- Synthesis Score: The synthesis score: 1, 2,3
- Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
- Design Notes and Details:
- GenBank File: A link to the GenBank file. file