EG10686

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Author Information

Sruthi Raguveer

Basic Information

  • ID: EG10686
  • Name: parC
  • Organism: E. coli
  • Description: parC codes for the alpha subunit of DNA topoisomerase IV in E. coli. DNA topoisomerase IV consists of two alpha subunits and two beta subunits. The beta subunits are coded for by parE. Topoisomerases are a vital group of proteins that deal with DNA topology. DNA topoisomerase IV aids mainly in regulating DNA topology during DNA replication UniProt. Well executed DNA replication is necessary for meiosis and cell reproduction. Topoisomerase IV ensures the decatenating, or unlinking, of newly replicated DNA strands and chromosomes. Separation of chromosomes is essential for chromosome segregation and proper cellular reproduction. Topoisomerase IV also relaxes supercoiled DNA. NIH.
  • DNA Length: 2259 base pairs.
  • DNA sequence:

ATG AGC GAT ATG GCA GAG CGC TTA GCT TTG CAC GAA TTC ACC GAA AAT GCT TAT CTT AAT TAT TCC ATG TAC GTG ATT ATG GAT CGC GCT TTG CCT TTC ATC GGC GAT GGA CTT AAA CCG GTG CAA CGT CGC ATT GTC TAC GCT ATG AGC GAA TTA GGG CTG AAT GCC TCC GCG AAA TTT AAA AAA AGC GCG CGC ACT GTT GGG GAT GTG CTG GGC AAA TAC CAC CCA CAC GGA GAC TCA GCT TGT TAT GAA GCA ATG GTC TTG ATG GCA CAG CCA TTC TCC TAC CGC TAC CCC TTA GTC GAT GGA CAA GGG AAT TGG GGC GCG CCT GAT GAT CCT AAA TCC TTT GCA GCT ATG CGC TAC ACA GAG TCT CGC CTT AGT AAG TAT TCT GAA TTG CTT TTA TCA GAA CTG GGA CAG GGC ACG GCT GAT TGG GTG CCG AAT TTT GAC GGA ACG TTA CAA GAG CCT AAG ATG CTG CCA GCG CGC CTT CCG AAC ATC TTA TTG AAT GGG ACT ACC GGC ATC GCT GTG GGG ATG GCA ACT GAT ATT CCT CCC CAT AAC CTG CGT GAA GTA GCT CAG GCG GCG ATT GCA TTA ATC GAT CAG CCA AAG ACA ACT CTT GAT CAG TTA CTT GAT ATT GTG CAA GGA CCT GAC TAT CCC ACT GAG GCA GAA ATT ATT ACC AGT CGC GCT GAG ATT CGT AAA ATT TAT GAG AAT GGT CGT GGG AGC GTG CGT ATG CGT GCC GTC TGG AAG AAG GAA GAC GGA GCC GTG GTT ATT TCG GCA TTA CCT CAT CAG GTC AGT GGG GCC CGT GTG TTG GAG CAG ATT GCT GCA CAA ATG CGC AAC AAG AAA CTG CCA ATG GTC GAC GAT CTT CGC GAT GAG TCA GAC CAT GAG AAT CCC ACG CGT CTT GTG ATC GTT CCC CGC TCC AAT CGT GTC GAT ATG GAC CAA GTG ATG AAC CAC CTG TTT GCT ACT ACT GAT CTG GAA AAA TCT TAC CGC ATC AAT TTG AAT ATG ATC GGT TTG GAC GGC CGT CCG GCA GTG AAG AAC TTG TTG GAG ATT TTA TCT GAG TGG TTG GTG TTT CGT CGC GAC ACT GTG CGT CGC CGT TTG AAT TAT CGC TTG GAG AAG GTG CTT AAA CGT CTG CAT ATC TTG GAG GGG CTG TTG GTG GCG TTC TTA AAT ATT GAT GAG GTT ATC GAA ATT ATT CGC AAC GAA GAC GAA CCT AAA CCT GCA TTG ATG TCC CGT TTT GGG CTT ACA GAA ACA CAG GCG GAA GCC ATC TTA GAA CTT AAA CTT CGC CAT TTA GCT AAG CTT GAA GAA ATG AAA ATT CGT GGC GAA CAA TCT GAG CTG GAA AAA GAG CGC GAC CAA TTA CAG GGC ATC TTA GCG TCC GAA CGT AAG ATG AAC AAT CTT CTG AAA AAG GAG CTT CAA GCC GAT GCT CAA GCT TAT GGG GAT GAC CGC CGT TCC CCT CTT CAA GAA CGT GAA GAG GCT AAA GCG ATG TCT GAA CAT GAT ATG CTG CCG TCG GAA CCA GTA ACC ATT GTT TTA TCG CAG ATG GGG TGG GTC CGT TCA GCT AAG GGT CAC GAT ATC GAC GCT CCT GGT CTT AAC TAC AAA GCG GGT GAT TCA TTT AAG GCG GCC GTC AAA GGG AAA TCC AAC CAA CCA GTG GTC TTT GTA GAC AGC ACC GGC CGT TCG TAT GCT ATC GAC CCG ATT ACA CTT CCT TCC GCA CGC GGA CAA GGC GAA CCT CTG ACC GGC AAA TTG ACC CTT CCG CCT GGC GCT ACT GTG GAT CAC ATG TTG ATG GAA TCC GAC GAT CAG AAA CTT CTT ATG GCT TCT GAT GCG GGA TAT GGA TTT GTG TGT ACC TTC AAT GAC TTA GTA GCC CGC AAC CGC GCT GGG AAG GCT TTG ATC ACG TTA CCG GAG AAT GCG CAC GTT ATG CCT CCG GTA GTG ATT GAA GAT GCT TCC GAT ATG TTG CTG GCC ATT ACC CAG GCA GGT CGT ATG CTT ATG TTC CCC GTA TCG GAC TTA CCC CAG TTA TCA AAG GGC AAG GGT AAT AAG ATT ATC AAC ATC CCT TCG GCG GAA GCG GCA CGT GGT GAA GAT GGT CTT GCA CAA CTG TAT GTC CTG CCG CCT CAG TCA ACC CTG ACG ATT CAC GTC GGA AAG CGC AAA ATC AAA CTT CGC CCA GAG GAA CTG CAA AAG GTC ACC GGC GAG CGT GGA CGC CGT GGC ACA TTG ATG CGT GGG TTA CAG CGT ATC GAT CGT GTA GAA ATC GAT AGC CCT CGT CGT GCA TCC TCC GGG GAC AGT GAG GAG TAA

  • Amino Acid length: 752 amino acids.
  • Amino Acid sequence:

MSDMAERLAL HEFTENAYLN YSMYVIMDRA LPFIGDGLKP VQRRIVYAMS ELGLNASAKF KKSARTVGDV LGKYHPHGDS ACYEAMVLMA QPFSYRYPLV DGQGNWGAPD DPKSFAAMRY TESRLSKYSE LLLSELGQGT ADWVPNFDGT LQEPKMLPAR LPNILLNGTT GIAVGMATDI PPHNLREVAQ AAIALIDQPK TTLDQLLDIV QGPDYPTEAE IITSRAEIRK IYENGRGSVR MRAVWKKEDG AVVISALPHQ VSGARVLEQI AAQMRNKKLP MVDDLRDESD HENPTRLVIV PRSNRVDMDQ VMNHLFATTD LEKSYRINLN MIGLDGRPAV KNLLEILSEW LVFRRDTVRR RLNYRLEKVL KRLHILEGLL VAFLNIDEVI EIIRNEDEPK PALMSRFGLT ETQAEAILEL KLRHLAKLEE MKIRGEQSEL EKERDQLQGI LASERKMNNL LKKELQADAQ AYGDDRRSPL QEREEAKAMS EHDMLPSEPV TIVLSQMGWV RSAKGHDIDA PGLNYKAGDS FKAAVKGKSN QPVVFVDSTG RSYAIDPITL PSARGQGEPL TGKLTLPPGA TVDHMLMESD DQKLLMASDA GYGFVCTFND LVARNRAGKA LITLPENAHV MPPVVIEDAS DMLLAITQAG RMLMFPVSDL PQLSKGKGNK IINIPSAEAA RGEDGLAQLY VLPPQSTLTI HVGKRKIKLR PEELQKVTGE RGRRGTLMRG LQRIDRVEID SPRRASSGDS EE

Function and Homologs

  • Product: DNA topoisomerase IV, A subunit
  • Closest homologous proteins:
    • DNA topoisomerase IV subunit A (Shigella flexneri 2a str. 301), 1540/100%/0/100%, NP_708834.1
    • DNA topoisomerase IV subunit A (Escherichia fergusonii), 1538/100%/0/99%, KTK74623.1
    • DNA topoisomerase IV subunit A (Shigella boydii Sb227), 1538/100%/0/99%, ABB67399.1

Expression

  • Expression Level: medium
  • Expression Level Hypothesis: parC is likely expressed at a medium level in E. coli because it codes for the alpha subunit of DNA Topoisomerase IV, a protein that is essential for chromosome segregation, a key step of cell division.
  • Expression Level References and Description: E. coli proteome data
  • Expression Time: late
  • Expression Time Hypothesis: As the protein this gene, parC, codes for is only really required to facilitate separation of the chromosomes after genome replication, it is only required for cellular division and thus can be expressed late in the reaction.
  • Expression Time References and Description: UniProt

Gene Context

  • Other Components: DNA Topoisomerase IV, B subunit. The B subunit of this protein is coded for by the parE gene. Two alpha subunits and two beta subunits form the complete DNA Topoisomerase IV enzyme
  • Possible Dependencies: DNA polymerases 1 and 3 are necessary for DNA replication and DNA Topoisomerase IV is active after replication.
  • Process: The main process DNA Topoisomerase IV takes part in is decatenating daughter strands following DNA replication. The enzyme takes the linked daughter strands and removes the linkages to have two separate DNA segments. This is crucial for chromosome segregation in cell division.
    • Inputs: Linked Daughter DNA strands, ATP
    • Outputs: Unlinked Daughter DNA strands
    • References: EcoCyc

Construct

  • Synthesis Score: The synthesis score of your construct: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details: For example, had to use a rare codon to fix folding energy;
  • GenBank File: A link to the GenBank file. file