- ID: EG10788
- Name: ptsH
- Organism: E. coli
- UniProt ID: EG10788 
- Description: The gene ptsH codes for HPr, a histidine and phosphocarrier protein, which plays an important part in sugar utilization. Specifically, it harbors the capacity to switch between the two alternative metabolic strategies available to the cell (hierarchical sugar utilization versus co-utilization), as part of the phosphoenolpyruvate-dependent phosphotransferase system (PTS)[]. HPr also has a regulatory role, coordinating carbon utilization strategy. Thus, by coding for a sugar active-transport system, ptsH is involved in an essential aspect of the organism’s survival: carbohydrate uptake and consumption. HPr, the protein this gene codes for, acts in partnership with phosphorylated Enzyme 1 and sugar-specific enzymes (Enzymes II) in the cell [].
- DNA Length: 258 base pairs.
- DNA sequence:
ATG TTC CAA CAG GAA GTT ACT ATT ACG GCC CCT AAC GGT TTA CAT ACA CGC CCT GCG GCT CAA TTC GTA AAG GAA GCG AAG GGA TTC ACG AGC GAA ATT ACT GTA ACG TCT AAC GGC AAG TCG GCT TCA GCC AAA TCT CTT TTC AAA TTA CAA ACA TTA GGA CTT ACG CAA GGC ACT GTG GTA ACA ATT TCC GCC GAA GGG GAG GAC GAA CAG AAG GCC GTC GAG CAC TTG GTC AAA TTG ATG GCT GAA TTG GAG TAA
- Amino Acid length: 85 amino acids.
- Amino Acid sequence:
MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV
VTISAEGEDE QKAVEHLVKL MAELE*
Function and Homologs
- Functional Category: Glucose transport & catabolism.
- Product: HPr (histidine protein)
- Module: Histidine biosynthesis.
- Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
- Equivalent E. coli / JCVI functional protein: MMSYN1_0694.
- Expression Level: High
- Expression Level Hypothesis: Just as in its homolog, because ptsH is involved in carbohydrate uptake and consumption, it plays a critical role in the survival of the organism. A high expression level for ptsH ensures continuous and effective sugar utilization via an active-transport system.
- Expression Level References and Description: This information is based on the E. coli Proteomic Expression dataset.
- Expression Time: Early
- Expression Level Hypothesis: ptsH must be produced early and often in the cell because it is responsible for managing metabolic pathways that provide the cell with energy. Without energy from sugar consumption, the cell cannot carry out more advanced functions.
- Expression Time References and Description: Resources used were the same as those cited in the “Basic Information/Description” section.
- Other Components: GlgP (Glycogen phosphorylase) []
- Possible Dependencies: PtsI
- PtsI-P: HPr, the protein that ptsH codes for, accepts a phosphoryl group from phosphorylated Enzyme I (PtsI-P).
- Enzymes II: HPr then transfers the phosphoryl group to sugar-specific enzymes (Enzymes II).
- Process: sugar utilization
- Inputs: sugar, cyclic AMP
- Outputs: PtsI, phosphoenolpyruvate
- Reference: Resources used were the same as those cited in the “Basic Information/Description” section.
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- Synthesis Score: The synthesis score: 1, 2,3
- Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
- Design Notes and Details:
- GenBank File: A link to the GenBank file. file