- ID: EG10789
- Name: ptsI
- Organism: E. Coli
- UniProt ID: 
- Description: Catalyzes the transfer of a phosphoryl group from PEP to HPr, which further catalyzes the phosphorylation of sugars (UniProt). Similar to the system described in the JVCI3.0 gene, the PTS system allows for the uptake of specific carbohydrates based on optimal conditions and can result in (Postma et al., 1993). While the enzymes may be slightly different, ptsI has the exact same function as hprK, but in gram-negative bacteria (Chauvin et. al Research in Microbiology 1996)
- DNA Length: 1728 base pairs.
- DNA sequence:
ATGATTTCAG GCATTTTAGC ATCCCCGGGT ATCGCTTTCG GTAAAGCTCT GCTTCTGAAA
GAAGACGAAA TTGTCATTGA CCGGAAAAAA ATTTCTGCCG ACCAGGTTGA TCAGGAAGTT
GAACGTTTTC TGAGCGGTCG TGCCAAGGCA TCAGCCCAGC TGGAAACGAT CAAAACGAAA
GCTGGTGAAA CGTTCGGTGA AGAAAAAGAA GCCATCTTTG AAGGGCATAT TATGCTGCTC
GAAGATGAGG AGCTGGAGCA GGAAATCATA GCCCTGATTA AAGATAAGCA CATGACAGCT
GACGCAGCTG CTCATGAAGT TATCGAAGGT CAGGCTTCTG CCCTGGAAGA GCTGGATGAT
GAATACCTGA AAGAACGTGC GGCTGACGTA CGTGATATCG GTAAGCGCCT GCTGCGCAAC
ATCCTGGGCC TGAAGATTAT CGACCTGAGC GCCATTCAGG ATGAAGTCAT TCTGGTTGCC
GCTGACCTGA CGCCGTCCGA AACCGCACAG CTGAACCTGA AGAAGGTGCT GGGTTTCATC
ACCGACGCGG GTGGCCGTAC TTCCCACACC TCTATCATGG CGCGTTCTCT GGAACTACCT
GCTATCGTGG GTACCGGTAG CGTCACCTCT CAGGTGAAAA ATGACGACTA TCTGATTCTG
GATGCCGTAA ATAATCAGGT TTACGTCAAT CCAACCAACG AAGTTATTGA TAAAATGCGC
GCTGTTCAGG AGCAAGTGGC TTCTGAAAAA GCAGAGCTTG CTAAACTGAA AGATCTGCCA
GCTATTACGC TGGACGGTCA CCAGGTAGAA GTATGCGCTA ACATTGGTAC GGTTCGTGAC
GTTGAAGGTG CAGAGCGTAA CGGCGCTGAA GGCGTTGGTC TGTATCGTAC TGAGTTCCTG
TTCATGGACC GCGACGCACT GCCCACTGAA GAAGAACAGT TTGCTGCTTA CAAAGCAGTG
GCTGAAGCGT GTGGCTCGCA AGCGGTTATC GTTCGTACCA TGGACATCGG CGGCGACAAA
GAGCTGCCAT ACATGAACTT CCCGAAAGAA GAGAACCCGT TCCTCGGCTG GCGCGCTATC
CGTATCGCGA TGGATCGTAG AGAGATCCTG CGCGATCAGC TCCGCGCTAT CCTGCGTGCC
TCGGCTTTCG GTAAATTGCG CATTATGTTC CCGATGATCA TCTCTGTTGA AGAAGTGCGT
GCACTGCGCA AAGAGATCGA AATCTACAAA CAGGAACTGC GCGACGAAGG TAAAGCGTTT
GACGAGTCAA TTGAAATCGG CGTAATGGTG GAAACACCGG CTGCCGCAAC AATTGCACGT
CATTTAGCCA AAGAAGTTGA TTTCTTTAGT ATCGGCACCA ATGATTTAAC GCAGTACACT
CTGGCAGTTG ACCGTGGTAA TGATATGATT TCACACCTTT ACCAGCCAAT GTCACCGTCC
GTGCTGAACT TGATCAAGCA AGTTATTGAT GCTTCTCATG CTGAAGGCAA ATGGACTGGC
ATGTGTGGTG AGCTTGCTGG CGATGAACGT GCTACACTTC TGTTGCTGGG GATGGGTCTG
GACGAATTCT CTATGAGCGC CATTTCTATC CCGCGCATTA AGAAGATTAT CCGTAACACG
AACTTCGAAG ATGCGAAGGT GTTAGCAGAG CAGGCTCTTG CTCAACCGAC AACGGACGAG
TTAATGACGC TGGTTAACAA GTTCATTGAA GAAAAAACAA TCTGCTAA
- Amino Acid length: 575 amino acids.
- Amino Acid sequence:
MISGILASPG IAFGKALLLK EDEIVIDRKK ISADQVDQEV ERFLSGRAKA SAQLETIKTK
AGETFGEEKE AIFEGHIMLL EDEELEQEII ALIKDKHMTA DAAAHEVIEG QASALEELDD
EYLKERAADV RDIGKRLLRN ILGLKIIDLS AIQDEVILVA ADLTPSETAQ LNLKKVLGFI
TDAGGRTSHT SIMARSLELP AIVGTGSVTS QVKNDDYLIL DAVNNQVYVN PTNEVIDKMR
AVQEQVASEK AELAKLKDLP AITLDGHQVE VCANIGTVRD VEGAERNGAE GVGLYRTEFL
FMDRDALPTE EEQFAAYKAV AEACGSQAVI VRTMDIGGDK ELPYMNFPKE ENPFLGWRAI
RIAMDRREIL RDQLRAILRA SAFGKLRIMF PMIISVEEVR ALRKEIEIYK QELRDEGKAF
DESIEIGVMV ETPAAATIAR HLAKEVDFFS IGTNDLTQYT LAVDRGNDMI SHLYQPMSPS
VLNLIKQVID ASHAEGKWTG MCGELAGDER ATLLLLGMGL DEFSMSAISI PRIKKIIRNT
NFEDAKVLAE QALAQPTTDE LMTLVNKFIE EKTIC
Function and Homologs
- Functional Category: Energy.
- Product: PTS Enzyme I
- Module: PTS
- Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
- Equivalent E. coli / JCVI functional protein: MMSYN1_0821
- Expression Level: High
- Expression Level Hypothesis: Carbohydrate uptake is an essential pathway that feeds into energy production cycles, such as glycolysis. As a result, ptsI must be expressed at a high level to catalyze this process at a sufficient level.
- Expression Level References and Description: Most of my information comes from literature review. There were a couple general overviews that I utilized (Chauvin et al. Research in Microbiology 1996, Potsma et al. 1993). From these papers, it was clear that the function of ptsI requires a high expression level.
- Expression Time: Right at Beginning
- Expression Level Hypothesis: The function of ptsI is to regulate the PTS system, which allows for the uptake of carbohydrates depending on different conditions. As a result of its function, it must be expressed immediately to allow for downstream pathways.
- Expression Time References and Description: Similar to the expression level, I used different general reviews of the PTS system and the enzyme itself to determine how essential it was to a cell.
- Other Components: HPr
- Possible Dependencies: cAMP (can control other sugar uptake pathways, leading to changes in the PTS system)
- Process: Glycolysis
- Inputs: Water, Glucose, Oxygen
- Outputs: ATP