EG10789

From BioE80 Boot
Jump to: navigation, search

Author Information

Anthony Agbay

Basic Information

  • ID: EG10789
  • Name: ptsI
  • Organism: E. Coli
  • UniProt ID: [1]
  • Description: Catalyzes the transfer of a phosphoryl group from PEP to HPr, which further catalyzes the phosphorylation of sugars (UniProt). Similar to the system described in the JVCI3.0 gene, the PTS system allows for the uptake of specific carbohydrates based on optimal conditions and can result in (Postma et al., 1993). While the enzymes may be slightly different, ptsI has the exact same function as hprK, but in gram-negative bacteria (Chauvin et. al Research in Microbiology 1996)
  • DNA Length: 1728 base pairs.
  • DNA sequence:

ATGATTTCAG GCATTTTAGC ATCCCCGGGT ATCGCTTTCG GTAAAGCTCT GCTTCTGAAA GAAGACGAAA TTGTCATTGA CCGGAAAAAA ATTTCTGCCG ACCAGGTTGA TCAGGAAGTT GAACGTTTTC TGAGCGGTCG TGCCAAGGCA TCAGCCCAGC TGGAAACGAT CAAAACGAAA GCTGGTGAAA CGTTCGGTGA AGAAAAAGAA GCCATCTTTG AAGGGCATAT TATGCTGCTC GAAGATGAGG AGCTGGAGCA GGAAATCATA GCCCTGATTA AAGATAAGCA CATGACAGCT GACGCAGCTG CTCATGAAGT TATCGAAGGT CAGGCTTCTG CCCTGGAAGA GCTGGATGAT GAATACCTGA AAGAACGTGC GGCTGACGTA CGTGATATCG GTAAGCGCCT GCTGCGCAAC ATCCTGGGCC TGAAGATTAT CGACCTGAGC GCCATTCAGG ATGAAGTCAT TCTGGTTGCC GCTGACCTGA CGCCGTCCGA AACCGCACAG CTGAACCTGA AGAAGGTGCT GGGTTTCATC ACCGACGCGG GTGGCCGTAC TTCCCACACC TCTATCATGG CGCGTTCTCT GGAACTACCT GCTATCGTGG GTACCGGTAG CGTCACCTCT CAGGTGAAAA ATGACGACTA TCTGATTCTG GATGCCGTAA ATAATCAGGT TTACGTCAAT CCAACCAACG AAGTTATTGA TAAAATGCGC GCTGTTCAGG AGCAAGTGGC TTCTGAAAAA GCAGAGCTTG CTAAACTGAA AGATCTGCCA GCTATTACGC TGGACGGTCA CCAGGTAGAA GTATGCGCTA ACATTGGTAC GGTTCGTGAC GTTGAAGGTG CAGAGCGTAA CGGCGCTGAA GGCGTTGGTC TGTATCGTAC TGAGTTCCTG TTCATGGACC GCGACGCACT GCCCACTGAA GAAGAACAGT TTGCTGCTTA CAAAGCAGTG GCTGAAGCGT GTGGCTCGCA AGCGGTTATC GTTCGTACCA TGGACATCGG CGGCGACAAA GAGCTGCCAT ACATGAACTT CCCGAAAGAA GAGAACCCGT TCCTCGGCTG GCGCGCTATC CGTATCGCGA TGGATCGTAG AGAGATCCTG CGCGATCAGC TCCGCGCTAT CCTGCGTGCC TCGGCTTTCG GTAAATTGCG CATTATGTTC CCGATGATCA TCTCTGTTGA AGAAGTGCGT GCACTGCGCA AAGAGATCGA AATCTACAAA CAGGAACTGC GCGACGAAGG TAAAGCGTTT GACGAGTCAA TTGAAATCGG CGTAATGGTG GAAACACCGG CTGCCGCAAC AATTGCACGT CATTTAGCCA AAGAAGTTGA TTTCTTTAGT ATCGGCACCA ATGATTTAAC GCAGTACACT CTGGCAGTTG ACCGTGGTAA TGATATGATT TCACACCTTT ACCAGCCAAT GTCACCGTCC GTGCTGAACT TGATCAAGCA AGTTATTGAT GCTTCTCATG CTGAAGGCAA ATGGACTGGC ATGTGTGGTG AGCTTGCTGG CGATGAACGT GCTACACTTC TGTTGCTGGG GATGGGTCTG GACGAATTCT CTATGAGCGC CATTTCTATC CCGCGCATTA AGAAGATTAT CCGTAACACG AACTTCGAAG ATGCGAAGGT GTTAGCAGAG CAGGCTCTTG CTCAACCGAC AACGGACGAG TTAATGACGC TGGTTAACAA GTTCATTGAA GAAAAAACAA TCTGCTAA

  • Amino Acid length: 575 amino acids.
  • Amino Acid sequence:

MISGILASPG IAFGKALLLK EDEIVIDRKK ISADQVDQEV ERFLSGRAKA SAQLETIKTK AGETFGEEKE AIFEGHIMLL EDEELEQEII ALIKDKHMTA DAAAHEVIEG QASALEELDD EYLKERAADV RDIGKRLLRN ILGLKIIDLS AIQDEVILVA ADLTPSETAQ LNLKKVLGFI TDAGGRTSHT SIMARSLELP AIVGTGSVTS QVKNDDYLIL DAVNNQVYVN PTNEVIDKMR AVQEQVASEK AELAKLKDLP AITLDGHQVE VCANIGTVRD VEGAERNGAE GVGLYRTEFL FMDRDALPTE EEQFAAYKAV AEACGSQAVI VRTMDIGGDK ELPYMNFPKE ENPFLGWRAI RIAMDRREIL RDQLRAILRA SAFGKLRIMF PMIISVEEVR ALRKEIEIYK QELRDEGKAF DESIEIGVMV ETPAAATIAR HLAKEVDFFS IGTNDLTQYT LAVDRGNDMI SHLYQPMSPS VLNLIKQVID ASHAEGKWTG MCGELAGDER ATLLLLGMGL DEFSMSAISI PRIKKIIRNT NFEDAKVLAE QALAQPTTDE LMTLVNKFIE EKTIC

Function and Homologs

  • Product: PTS Enzyme I
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • HPr(Ser) kinase/phosphatase [Mycoplasma mycoides], 632/100%/0/100%, [2]
    • HPr kinase/phosphatase [Mycoplasma leachii], 612/100%/0/96%, [3]
    • HPr(Ser) kinase/phosphatase [Mycoplasma capricolum], 608/100%/0/95%, [4]
  • Equivalent E. coli / JCVI functional protein: MMSYN1_0821

Expression

  • Expression Level: High
  • Expression Level Hypothesis: Carbohydrate uptake is an essential pathway that feeds into energy production cycles, such as glycolysis. As a result, ptsI must be expressed at a high level to catalyze this process at a sufficient level.
  • Expression Level References and Description: Most of my information comes from literature review. There were a couple general overviews that I utilized (Chauvin et al. Research in Microbiology 1996, Potsma et al. 1993). From these papers, it was clear that the function of ptsI requires a high expression level.
  • Expression Time: Right at Beginning
  • Expression Level Hypothesis: The function of ptsI is to regulate the PTS system, which allows for the uptake of carbohydrates depending on different conditions. As a result of its function, it must be expressed immediately to allow for downstream pathways.
  • Expression Time References and Description: Similar to the expression level, I used different general reviews of the PTS system and the enzyme itself to determine how essential it was to a cell.

Gene Context

  • Other Components: HPr
  • Possible Dependencies: cAMP (can control other sugar uptake pathways, leading to changes in the PTS system)
  • Process: Glycolysis
    • Inputs: Water, Glucose, Oxygen
    • Outputs: ATP