EG10831 msnelson

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Author Information

Meredith Nelson SUID: msnelson

Basic Information

  • ID: EG10831
  • Name: reco: DNA repair protein RecO
  • Organism: E. coli
  • UniProt ID: P05824 [1]
  • Description: This is the E coli equivalent of the gene MMSYN1_0404 used in the DNA repair of the protein RecO, MMSYN1_0404. Although this gene was identified as a DNA repair protein RecO (most likely due to generic testing methods), it is slightly more homologous to RecN (21.3% alignment versus 17.0% alignment for RecO). These differences in alignment are slight and make it difficult to determine which is for certain the homolog. This E. Coli page is for RecN since it represents slightly better alignment. Below are descriptions of both RecO and RecN.
    • RecO is a component of the RecFOR complex which functions in RecA-mediated replication recovery [Matsunaga96]. Citations found on [2]
    • RecN is required for repair of double strand breaks in the chromosome when these breaks occur at two or more locations, but not when there is a single break in the chromosome. DksA works with RecN in this capacity. A recN dksA double mutant becomes filamentous and is unable to divide after induction of double-strand breaks [Meddows05]. recN mutations result in increased sensitivity to Mitomycin C and ionizing radiation correlated with reduced UV inducible repair of double strand breaks in DNA [Picksley84]. Multiple copies of recN increased resistance of recN mutants to ionizing radiation and decreased survival of wild-type cells [Picksley85]. RecN is important for bleomycin lesion repair [Kosa04]. RecN has been implicated in the protection of DNA that is afforded by the clinical radioprotector amifostine [Almeida10]. All citations found on [3]
  • DNA Length: 1662 base pairs
  • DNA sequence:

ATG CTG GCT CAG TTA ACA ATT TCT AAC TTT GCG ATT GTA CGC GAG CTG GAA ATC GAC TTC CAC TCG GGG ATG ACG GTA ATT ACA GGT GAA ACT GGC GCC GGC AAG AGT ATT GCA ATC GAC GCA TTG GGA CTG TGC CTT GGA GGA CGC GCC GAA GCA GAC ATG GTG CGT ACC GGT GCG GCC CGC GCG GAT CTT TGT GCT CGT TTC AGC TTA AAG GAC ACT CCG GCG GCT TTA CGT TGG TTA GAA GAG AAT CAG TTA GAG GAT GGA CAT GAA TGC CTG TTG CGC CGC GTT ATT TCC TCA GAT GGC CGT AGT CGT GGT TTT ATT AAT GGG ACC GCC GTT CCC TTG TCG CAA TTA CGC GAG CTT GGG CAG TTG CTG ATT CAA ATT CAT GGG CAA CAC GCA CAC CAA TTA TTA ACC AAA CCC GAG CAC CAG AAG TTC TTG CTG GAT GGG TAT GCA AAT GAA ACG AGT TTG CTT CAG GAA ATG ACT GCC CGT TAC CAG TTG TGG CAC CAG TCA TGC CGC GAT TTA GCA CAC CAT CAA CAA TTA AGC CAA GAA CGC GCG GCA CGC GCC GAG CTG TTA CAG TAC CAG CTG AAG GAA TTA AAT GAA TTC AAC CCC CAG CCC GGC GAG TTT GAG CAA ATT GAT GAA GAA TAC AAG CGC TTA GCT AAT TCA GGT CAA CTT CTT ACA ACT TCC CAG AAT GCA CTG GCT TTG ATG GCT GAC GGA GAA GAT GCG AAC TTG CAG TCC CAG CTT TAC ACA GCG AAG CAA TTG GTG TCC GAG TTA ATC GGA ATG GAC TCT AAG CTT AGC GGC GTT TTG GAC ATG CTG GAA GAG GCT ACT ATT CAA ATC GCT GAG GCT TCC GAT GAA TTA CGT CAC TAC TGT GAC CGT CTT GAT CTG GAT CCT AAC CGT CTG TTC GAG CTG GAA CAG CGT ATT TCC AAG CAG ATC TCT TTG GCT CGT AAA CAC CAC GTA AGT CCG GAA GCC CTG CCA CAG TAT TAT CAA TCG TTA CTG GAA GAG CAG CAA CAA CTT GAC GAT CAG GCG GAC TCA CAG GAA ACA CTT GCG CTT GCT GTC ACG AAA CAT CAC CAA CAG GCC CTG GAA ATC GCG CGT GCG TTA CAT CAG CAA CGC CAA CAA TAC GCT GAA GAA TTG GCT CAA TTA ATT ACT GAT TCT ATG CAC GCA CTG AGT ATG CCG CAT GGT CAA TTC ACT ATT GAT GTG AAG TTC GAC GAG CAC CAT CTG GGA GCC GAC GGG GCT GAC CGC ATT GAG TTC CGC GTG ACC ACT AAT CCT GGT CAG CCG ATG CAG CCG ATT GCA AAA GTT GCA TCG GGA GGT GAG CTG TCA CGC ATT GCC TTA GCA ATC CAG GTG ATT ACA GCA CGC AAG ATG GAG ACA CCT GCC TTA ATC TTT GAC GAA GTG GAT GTA GGA ATT TCG GGT CCT ACC GCG GCT GTG GTT GGT AAG CTT CTT CGC CAG CTT GGT GAG TCA ACG CAG GTA ATG TGC GTG ACG CAT CTG CCT CAA GTG GCG GGG TGT GGG CAC CAG CAC TAC TTC GTA AGC AAA GAA ACT GAT GGA GCT ATG ACA GAA ACT CAC ATG CAG TCA CTG AAC AAA AAG GCT CGT TTG CAA GAA CTT GCG CGT CTG CTG GGG GGG AGC GAA GTT ACC CGT AAT ACT TTG GCA AAT GCT AAG GAA TTA CTG GCG GCA TAA

  • Amino Acid length: 553 amino acids
  • Amino Acid sequence:

MLAQLTISNF AIVRELEIDF HSGMTVITGE TGAGKSIAID ALGLCLGGRA EADMVRTGAA RADLCARFSL KDTPAALRWL EENQLEDGHE CLLRRVISSD GRSRGFINGT AVPLSQLREL GQLLIQIHGQ HAHQLLTKPE HQKFLLDGYA NETSLLQEMT ARYQLWHQSC RDLAHHQQLS QERAARAELL QYQLKELNEF NPQPGEFEQI DEEYKRLANS GQLLTTSQNA LALMADGEDA NLQSQLYTAK QLVSELIGMD SKLSGVLDML EEATIQIAEA SDELRHYCDR LDLDPNRLFE LEQRISKQIS LARKHHVSPE ALPQYYQSLL EEQQQLDDQA DSQETLALAV TKHHQQALEI ARALHQQRQQ YAEELAQLIT DSMHALSMPH GQFTIDVKFD EHHLGADGAD RIEFRVTTNP GQPMQPIAKV ASGGELSRIA LAIQVITARK METPALIFDE VDVGISGPTA AVVGKLLRQL GESTQVMCVT HLPQVAGCGH QHYFVSKETD GAMTETHMQS LNKKARLQEL ARLLGGSEVT RNTLANAKEL LAA

Function and Homologs

  • Functional Category: DNA repair
  • Product: Recombination and DNA repair protein
  • Module: uncertain DNA repair
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • DNA repair protein RecN [Escherichia coli MS 107-1], Max score = 1131 / Query Cover = 100% / E-Value = 0.0 / Ident = 100%, [4]
    • MULTISPECIES: DNA repair protein RecN [Proteobacteria], Max score = 1131 / Query Cover = 100% / E-Value = 0.0 / Ident = 100%, [5]
    • recombination and repair protein [Escherichia coli K-12], Max score = 1130 / Query Cover = 100% / E-Value = 0.0 / Ident = 100%, [6]
  • Equivalent JCVI functional protein: MMSYN1_0404 [7]

Expression

  • Expression Level: Low
  • Expression Level Hypothesis: The expression level for DNA Repair is usually low to medium. This is backed up the spreadsheet confirming the low expression of RecN.
  • Expression Level References and Description: The expression level was determined by finding the uniprot ID of the E. coli for RecN in the spreadsheet cited and using the associated expression level [8] More on general expression levels in DNA repair genes was found at [9]
  • Expression Time: Anytime during the organism lifecycle
  • Expression Level Hypothesis: DNA repair must be able to occur at any point in the organism's lifecycle because issues can arise at anytime.
  • Expression Time References and Description: Understanding of different stages of DNA repair in an organism's lifecycle are outlined in Nature in the review 'Regulation of DNA repair throughout the cell cycle' [10]

Gene Context

  • Other Components: RecA is a component, found in its E coli equivalent (the specific page was not created on the wiki for this gene) The RecR recombination protein E coli equivalent EG10834 [11] also has RecA as a component, meaning it is a component of this gene as well
  • Possible Dependencies: amino acid biosynthesis, ATP synthesis, nucleotide synthesis, protein synthesis
  • Process: DNA repair
    • Inputs: Damaged DNA template, enzyme that does that does repair, free nucleotides, energy (ATP)
    • Outputs: Repaired DNA