EG10834 msnelson

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Author Information

Meredith Nelson SUID: msnelson

Basic Information

  • ID: EG10834
  • Name: recR: recombination protein RecR
  • Organism: E. coli
  • UniProt ID: P0A7H6 [1]
  • Description: This is the equivalent E. coli homolog for the gene MMSYN1_0046. RecR is a component of the RecFOR complex which functions in RecA-mediated replication recovery. Citation in [2]
  • DNA Length: 606 base pairs
  • DNA sequence:

ATG CAG ACC AGT CCG TTG CTT ACT CAG TTA ATG GAG GCC TTA CGT TGT TTA CCT GGA GTC GGC CCC AAG TCC GCG CAA CGC ATG GCC TTC ACA TTA TTG CAA CGT GAC CGC TCC GGG GGC ATG CGC TTG GCG CAA GCT CTG ACT CGC GCA ATG TCC GAG ATC GGA CAC TGT GCG GAC TGT CGC ACC TTT ACT GAG CAA GAA GTT TGT AAC ATT TGC TCA AAC CCC CGT CGC CAG GAA AAC GGA CAA ATC TGT GTA GTA GAG TCC CCG GCT GAC ATC TAT GCC ATC GAG CAA ACC GGA CAA TTC AGT GGC CGC TAT TTC GTC CTG ATG GGG CAC TTG TCT CCT CTT GAC GGT ATC GGA CCA GAT GAT ATT GGA CTG GAC CGT CTG GAG CAG CGC CTG GCA GAA GAG AAA ATC ACG GAG GTT ATT TTA GCG ACG AAC CCG ACC GTG GAG GGT GAA GCG ACG GCT AAT TAT ATT GCC GAG TTA TGT GCC CAA TAC GAC GTT GAA GCC AGT CGT ATC GCG CAT GGC GTT CCA GTG GGG GGT GAA TTG GAG ATG GTT GAC GGG ACT ACA TTG TCG CAC TCG TTA GCG GGA CGT CAC AAA ATC CGT TTT TAA

  • Amino Acid length: 201 amino acids
  • Amino Acid sequence:

MQTSPLLTQL MEALRCLPGV GPKSAQRMAF TLLQRDRSGG MRLAQALTRA MSEIGHCADC RTFTEQEVCN ICSNPRRQEN GQICVVESPA DIYAIEQTGQ FSGRYFVLMG HLSPLDGIGP DDIGLDRLEQ RLAEEKITEV ILATNPTVEG EATANYIAEL CAQYDVEASR IAHGVPVGGE LEMVDGTTLS HSLAGRHKIR F

Function and Homologs

  • Functional Category: DNA repair
  • Product: recombination and repair
  • Module: RECFOR
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • MULTISPECIES: recombination protein RecR [Proteobacteria], Max score = 414 / Query Cover = 100% / E-Value = 2e-146 / Ident = 100% , [3]
    • recombination protein RecR [Escherichia coli], Max score = 413 / Query Cover = 100% / E-Value = 3e-146/ Ident = 99% , [4]
    • recombination protein RecR [Escherichia coli], Max score = 413 / Query Cover = 100% / E-Value = 3e-146 / Ident = 99% , [5]
  • Equivalent JCVI functional protein: MMSYN1_0046 [6]

Expression

  • Expression Level: Medium
  • Expression Level Hypothesis: The expression level for recombination is usually medium. This is backed up by the spreadsheet confirming the medium expression of RecR
  • Expression Level References and Description: The expression level was determined by finding the uniprot ID of the E. coli for RecR in the spreadsheet cited and using the associated expression level [7]
  • Expression Time: Anytime during the organism lifecycle
  • Expression Level Hypothesis: Recombination must be able to occur at any point in the organism's lifecycle because issues can arise at anytime
  • Expression Time References and Description: Information about DNA recombination expression and timing is found at NCBI [8]

Gene Context

  • Other Components: RecA is a component, found in its E coli equivalent (the specific page was not created on the wiki for this gene) The RecO DNA repair protein E coli equivalent EG10831 [9] also has RecA as a component, meaning it is a component of this gene as well
  • Possible Dependencies: amino acid biosynthesis, ATP synthesis, nucleotide synthesis, protein synthesis
  • Process: Recombination
    • Inputs: DNA template, enzyme that does that does recombination (ligase), free nucleotides, enzyme to seal the ends of the DNA, energy (ATP), cofactors (magnesium, other salts that help the enzymes work)
    • Outputs: Recombined DNA