EG10869

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Author Information

Maria Gutierrez

Basic Information

  • ID: EG10869
  • Name: rplF
  • Organism: E. coli'
  • Description: This protein binds directly to at least 2 domains of the 23S ribosomal RNA, thus is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center. UniProt. The L6 protein is a component of the 50S subunit of the ribosome and is involved in the late stage assembly of 50S ribosomal subunits. L6 interacts with 23S rRNA. Interaction appears to be cooperative with L3. L6 crosslinks to the stem-loop structure in domain V of 23S rRNA, but assembles to domain VI. Ecocyc
  • DNA Length: 534
  • DNA sequence:

ATG TCT CGC GTG GCT AAA GCT CCA GTG GTG GTT CCA GCT GGC GTA GAT GTA AAG ATC AAC GGG CAG GTT ATT ACT ATC AAG GGC AAA AAC GGC GAA TTA ACC CGT ACA CTG AAT GAT GCC GTG GAA GTT AAG CAT GCA GAT AAT ACT TTA ACC TTC GGA CCA CGC GAT GGC TAT GCC GAC GGA TGG GCC CAA GCG GGT ACC GCA CGC GCG CTG CTG AAT AGT ATG GTA ATC GGG GTA ACC GAA GGA TTT ACA AAA AAA TTG CAA CTT GTT GGA GTA GGT TAT CGT GCC GCA GTC AAG GGC AAC GTA ATC AAT TTA TCC CTG GGC TTT TCA CAC CCT GTG GAT CAC CAG TTA CCA GCA GGC ATC ACG GCT GAA TGC CCG ACA CAG ACA GAG ATT GTA TTA AAG GGG GCA GAC AAG CAG GTT ATC GGT CAA GTT GCT GCC GAC CTG CGT GCA TAT CGT CGC CCT GAA CCC TAC AAA GGT AAA GGG GTA CGC TAT GCC GAC GAA GTG GTT CGC ACC AAG GAA GCG AAA AAA AAG TAA

  • Amino Acid length: 177
  • Amino Acid sequence:

MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAKKK

Function and Homologs

  • Product: 50S ribosomal protein L6
  • Module: Ribosome (This protein binds to the 23S rRNA, and is important in its secondary structure.)
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • MULTISPECIES: 50S ribosomal protein L6 [Proteobacteria], 358/100%/3e-127/100%, WP_000091945.1
    • 50S ribosomal protein L6 [Escherichia coli], 358/100%/3e-127/99%, WP_077581172.1
    • MULTISPECIES: 50S ribosomal protein L6 [Escherichia], 358/100%/3e-127/99%, WP_000091934.1
  • Equivalent E. coli / JCVI functional protein: MMSYN1_0656.


Expression

  • Expression Level: High.
  • Expression Level Hypothesis: Because this protein is involved in translation, then it must be highly expressed in order for it to be of aid in all of the translation the cell needs to do. It is involved in the assembly of the subunits of the ribosome, and thus is required for every single translation that occurs within the cell, and thus, vital for all its functionality.
  • Expression Level References and Description: In general, I looked at the main websites that have previously been used in class such as NCBI, UniProt, EcoCyc. I also googled my gene and its expression level and arrived at different articles that describe how L6 is important for the assembly of the ribosomal subunit NCBI and thus its expression is high.
  • Expression Time: Early
  • Expression Level Hypothesis: L6 is used in translation, which occurs during interphase (the first stage of the cell cycle). Since it is required for assembly of the ribosome subunits, then I hypothesized it occurs early on.
  • Expression Time References and Description: Googling about when translation occurs in the cell I found it it's one of the main components of interphase as well as happening throughout the cell cycle. After discussing with teachers and peers, I came to the conclusion that since L6 is required to assemble the ribosome subunits, and ribosomes are necessary since the beginning of the lifecycle of the cell, its expression time starts since the beginning of the cell.

Gene Context

  • Other Components: rplI (polypeptide: 50S ribosomal protein L9) MMSYN1 0833
  • Possible Dependencies: RNA polymerase delta subunit is involved in transcribing DNA, and the ribosome then enters and transcribes what the RNA Polymerase created. Thus the ribosome depends on the RNA Polymerase doing its job in order to be able to transcribe the mRNA.
  • Process: Translation Initiation
    • Inputs: Charged tRNA, Ribosomal subunits, mRNA, GTP, Initiation factors (IF1, IF2, IF3)
    • Outputs: Initiation complex, GDP