EG10880

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Author Information

Blake Salvador

Basic Information

  • ID: EG10880
  • Name: rplS (Ribosomal protein L19)
  • Organism: E. coli
  • Description:

Ribosomes are the organelles in the cell responsible for translation of mRNA into a protein. Bacterial ribosomes are made of two subunits - the smaller 30S subunit and the larger 50S subunit - as well as ribosomal RNA (rRNA) held together by the proteins. This gene encodes a protein component of the 50S subunit known as ribosomal protein L19. [1]

L19 has both RNA chaperone-like activity and protein chaperone activity. The effects of mutations vary, but include a slow growth phenotype and unbalanced synthesis of RNA. [2]

L19 belongs to the L19E family of ribosomal proteins, and is located in the cytoplasm. [3]

  • DNA Length: 348 base pairs.
  • DNA sequence:

ATG TCG AAC ATC ATC AAA CAG TTA GAA CAG GAG CAG ATG AAA CAA GAT GTT CCT TCC TTC CGC CCA GGA GAC ACA GTG GAA GTG AAG GTT TGG GTC GTC GAA GGG TCA AAG AAA CGC CTT CAG GCG TTT GAA GGT GTC GTT ATT GCT ATT CGC AAT CGT GGA TTA CAT AGT GCT TTC ACT GTC CGC AAG ATC AGC AAC GGA GAA GGA GTC GAG CGC GTT TTT CAA ACC CAT TCG CCG GTT GTC GAT TCG ATT TCC GTG AAA CGT CGT GGA GCT GTA CGC AAG GCG AAG TTA TAT TAT CTG CGT GAA CGC ACC GGT AAA GCA GCC CGT ATT AAG GAA CGT TTG AAT TGA

  • Amino Acid length: 115 amino acids.
  • Amino Acid sequence:

MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRERTGKAARIKERLN

Function and Homologs

  • Product: Ribosomal Protein L19
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • 50S ribosomal protein L19 (Proteobacteria), 232/100%/2e-77/100%, [WP_000065253.1]
    • 50S ribosomal protein L19 (Enterobacteriaceae), 231/100%/3e-77/99%, [WP_000065254.1]
    • 50S ribosomal protein L19 (Gammaproteobacteria), 231/100%/4e-77/99%, [WP_012134695.1]

Expression

  • Expression Level: High. At 16521, the quantitative expression score is significantly higher than many other proteins.
  • Expression Level Hypothesis: I hypothesize that the expression level is high because ribosomes are absolutely essential for proper cellular function and are a huge percentage of the cell's mass (an E. coli cell contains 20,000 ribosomes which constitute 25% of the cell's total mass: http://bscb.org/learning-resources/softcell-e-learning/ribosome/). Thus, to ensure that a steady supply of them is in the cell, expression of all subunits must remain high.
  • Expression Level References and Description: Schmidt's quantitative measures of protein expression in E. coli: File:MgenitaliumSimProteinCounts.xlsx. Also, the page linked above in "Expression Level Hypothesis".
  • Expression Time: Right at beginning.
  • Expression Level Hypothesis: Ribosomes are absolutely essential for bacterial cells, and constitute a huge percentage of the cell mass. The high expression level seems to indicate that there is a high degradation rate of ribosomes, and so to maintain a steady supply a cell would need to express proteins for them right at the beginning.
  • Expression Time References and Description: See above link in "expression level hypothesis" for where I read about bacterial ribosomes.

Gene Context

  • Other Components: G10908 --> Ribosomal protein rpsl, a component of the 30S subunit.
  • Possible Dependencies: EG10270 --> Essential for rRNA production, which is itself essential for ribosome formation.
  • Process: 16S rRNA binding to stabilize ribosome
    • Inputs: 16S rRNA, ribosomal protein L19
    • Outputs: 16S rRNA-ribosomal protein L19 complex

Construct

We will handle this - not part of your assignment

  • Synthesis Score: The synthesis score: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details:
  • GenBank File: A link to the GenBank file. file