EG11033

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Author Information

Noor Siddiqui

Basic Information

  • ID: EG11033
  • Name: tsf
  • Organism: E. coli
  • Description: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome[1].
  • DNA Length: 852 base pairs.
  • DNA sequence:

ATG GCA GAG ATC ACA GCA TCG TTG GTT AAG GAG CTT CGT GAG CGT ACA GGC GCT GGT ATG ATG GAT TGC AAA AAA GCT CTG ACC GAA GCA AAT GGA GATATC GAA TTA GCG ATT GAG AAC ATG CGT AAA TCT GGT GCG ATT AAA GCA GCA AAG AAG GCT GGG AAT GTT GCC GCA GAT GGG GTT ATT AAA ACA AAA ATTGAC GGC AAT TAT GGG ATC ATT CTG GAA GTG AAC TGC CAA ACA GAC TTC GTT GCA AAG GAT GCT GGT TTT CAA GCG TTT GCA GAC AAG GTC TTG GAT GCAGCG GTT GCG GGA AAA ATC ACA GAT GTA GAG GTT TTA AAG GCT CAG TTT GAG GAG GAA CGT GTG GCC TTA GTA GCG AAA ATT GGC GAG AAC ATC AAT ATCCGT CGC GTC GCC GCC CTT GAG GGG GAC GTG TTG GGG TCA TAT CAA CAT GGT GCG CGT ATT GGT GTT CTT GTG GCC GCA AAA GGT GCT GAT GAA GAG CTTGTT AAG CAT ATC GCT ATG CAC GTC GCT GCC TCG AAG CCC GAG TTC ATC AAG CCT GAA GAC GTT TCA GCA GAG GTT GTG GAG AAG GAA TAT CAA GTC CAACTT GAC ATT GCG ATG CAA TCC GGT AAG CCT AAA GAG ATC GCC GAG AAG ATG GTG GAG GGC CGC ATG AAA AAA TTC ACT GGG GAG GTG AGC CTT ACT GGACAA CCG TTC GTT ATG GAG CCC TCG AAA ACA GTG GGA CAA CTT TTG AAG GAG CAC AAC GCA GAA GTT ACT GGG TTT ATT CGT TTC GAA GTT GGT GAA GGTATT GAG AAA GTT GAA ACC GAT TTT GCT GCG GAG GTA GCA GCT ATG AGC AAG CAG TCA TAA

  • Amino Acid length: 287 amino acids.
  • Amino Acid sequence:

MAEITASLVK ELRERTGAGM MDCKKALTEA NGDIELAIEN MRKSGAIKAA KKAGNVAADG VIKTKIDGNY GIILEVNCQT DFVAKDAGFQ AFADKVLDAA VAGKITDVEV LKAQFEEERV ALVAKIGENI NIRRVAALEG DVLGSYQHGA RIGVLVAAKG ADEELVKHIA MHVAASKPEF IKPEDVSAEV VEKEYQVQLD IAMQSGKPKE IAEKMVEGRM KKFTGEVSLT GQPFVMEPSK TVGQLLKEHN AEVTGFIRFE VGEGIEKVET DFAAEVAAMS KQS

Function and Homologs

  • Product: Elongation factor Ts
  • Module: Ribosome
  • Closest homologous proteins:
    • translation elongation factor Ts [Shigella boydii] 559/559/100%/0.0/99% [2]
    • MULTISPECIES: elongation factor Ts [Enterobacteriaceae] 553/553/100%/0.0/98% [3]
    • elongation factor Ts [Salmonella bongori] 547/547/100%/0.0/97% [4]
  • Equivalent E. coli / JCVI functional protein: MMSYN1 0539.

Expression

  • Expression Level: High
  • Expression Level Hypothesis: A lot of translation needs to occur, so it makes sense that elongation factors would also need to be expressed at a high level to facilitate translation.
  • Expression Level References and Description: excel spreadsheet
  • Expression Time: Right at beginning
  • Expression Level Hypothesis: Based on the central dogma, it is necessary for translation to occur in order for proteins to be produced
  • Expression Time References and Description: [5]

Gene Context

  • Other Components in the functional module: 50S ribosomal protein L6 EG10869
  • Possible Dependencies: MMSYN1 0128The ribosome depends on RNA polymerase functioning in order to be able to transcribe the mRNA.
  • Process: Translation Initiation
    • Inputs:tRNA, ribosomal subunits, initiation factors - IF1, IF2, IF3, mRNA, GTP
    • Outputs: initiation complex, GDP

Construct

We will handle this - not part of your assignment

  • Synthesis Score: The synthesis score: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details:
  • GenBank File: A link to the GenBank file. file