EG11036

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Author Information

Eleanor Glockner

Basic Information

  • ID: EG11036
  • Name: EF-Tu: translation elongation factor Tu
  • Organism: JCVI-Syn3.0
  • UniProt ID: P0CE47 [1]
  • Description: This gene encodes a protein that assists in tRNA binding to the ribosome. If the cell is infected by bacteriophage Qbeta, the protein will become part of the viral RNA-dependent RNA polymerase complex. Source: [2]
  • DNA Length: 1185 base pairs.
  • DNA sequence:

gtgTCTAAAG AAAAATTTGA ACGTACAAAA CCGCACGTTA ACGTTGGTAC TATCGGCCAC GTTGACCACG GTAAAACTAC TCTGACCGCT GCAATCACCA CCGTACTGGC TAAAACCTAC GGCGGTGCTG CTCGTGCATT CGACCAGATC GATAACGCGC CGGAAGAAAA AGCTCGTGGT ATCACCATCA ACACTTCTCA CGTTGAATAC GACACCCCGA CCCGTCACTA CGCACACGTA GACTGCCCGG GGCACGCCGA CTATGTTAAA AACATGATCA CCGGTGCTGC TCAGATGGAC GGCGCGATCC TGGTAGTTGC TGCGACTGAC GGCCCGATGC CGCAGACTCG TGAGCACATC CTGCTGGGTC GTCAGGTAGG CGTTCCGTAC ATCATCGTGT TCCTGAACAA ATGCGACATG GTTGATGACG AAGAGCTGCT GGAACTGGTT GAAATGGAAG TTCGTGAACT TCTGTCTCAG TACGACTTCC CGGGCGACGA CACTCCGATC GTTCGTGGTT CTGCTCTGAA AGCGCTGGAA GGCGACGCAG AGTGGGAAGC GAAAATCCTG GAACTGGCTG GCTTCCTGGA TTCTTATATT CCGGAACCAG AGCGTGCGAT TGACAAGCCG TTCCTGCTGC CGATCGAAGA CGTATTCTCC ATCTCCGGTC GTGGTACCGT TGTTACCGGT CGTGTAGAAC GCGGTATCAT CAAAGTTGGT GAAGAAGTTG AAATCGTTGG TATCAAAGAG ACTCAGAAGT CTACCTGTAC TGGCGTTGAA ATGTTCCGCA AACTGCTGGA CGAAGGCCGT GCTGGTGAGA ACGTAGGTGT TCTGCTGCGT GGTATCAAAC GTGAAGAAAT CGAACGTGGT CAGGTACTGG CTAAGCCGGG CACCATCAAG CCGCACACCA AGTTCGAATC TGAAGTGTAC ATTCTGTCCA AAGATGAAGG CGGCCGTCAT ACTCCGTTCT TCAAAGGCTA CCGTCCGCAG TTCTACTTCC GTACTACTGA CGTGACTGGT ACCATCGAAC TGCCGGAAGG CGTAGAGATG GTAATGCCGG GCGACAACAT CAAAATGGTT GTTACCCTGA TCCACCCGAT CGCGATGGAC GACGGTCTGC GTTTCGCAAT CCGTGAAGGC GGCCGTACCG TTGGCGCGGG CGTTGTTGCT AAAGTTCTGG GCtaa

  • Amino Acid length: 400 amino acids.
  • Amino Acid sequence:

MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAARAFDQIDNAPEEKARG ITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHI LLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALE GDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVG EEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIK PHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMV VTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG

Function and Homologs

  • Product: Elongation factor Tu 1
  • Closest homologous proteins:
    • Chain A (Qb replicase), 807/99%/0.0/100% [3]
    • Elongation factor Tu (bacteria, multispecies), 797/100%/0.0/100% [4]
    • Elongation factor Tu (E. coli), 797/100%/0.0/99%, [5]

Expression

  • Expression Level: high
  • Expression Level Hypothesis: The protein produced by this gene aids in translation, which is an important process essential for cell function. According to EcoCyc, this gene produces the most abundant protein in E. coli.
  • Expression Level References and Description: E. coli proteomic expression data [6]; EcoCyc [7]
  • Expression Time: right at beginning
  • Expression Level Hypothesis: Because this gene helps facilitate translation, it is important for it to be expressed early to aid in biosynthesis of essential proteins.
  • Expression Time References and Description: EcoCyc [8]


Gene Context

  • Other Components: ribosome, tRNA
  • Possible Dependencies: GTP
  • Process: translation
    • Inputs: mRNA, tRNA
    • Outputs: proteins coded by mRNA