EG11831

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Author Information

Camila Camacho

Basic Information

  • ID: EG11831
  • Name: srkA
  • Organism: E. coli
  • Description: SrkA is a serine/threonine protein kinase that protects cells from several types of lethal stress Zheng07. A serine/threonine protein kinase is a kinase enzyme that phosphorylates the OH group of serine or threonine (which have similar sidechains). Serine/threonine protein kinase describes a class of enzymes that belong to the family of transferases, specifically protein-serine/threonine kinases. These enzymes transfer phosphates to the oxygen atom of a serine or threonine sidechain in proteins. This process is called phosphorylation. Protein phosphorylation in particular plays a significant role in a wide range of cellular processes and is a very important posttranslational modification. The chemical reaction performed by these enzymes can be written as ATP + a protein -> ADP + a phosphoprotein. Thus, the two substrates of this enzyme are ATP and a protein, whereas its two products are ADP and phosphoprotein. SrkA is able to autophosphorylate at serine and threonine, but not tyrosine residues Zheng07. The protein kinase activity is required for stress protection by SrkA DorseyOresto13. The enzyme probably acts to suppress the effects of stress linked to accumulation of reactive oxygen species. It protects cells from stress by antagonizing the MazE-MazF TA module, probably indirectly as it has not been seen to phosphorylate MazE, MazF or MazG. It is probably involved in the extracytoplasmic stress response. SrkA is also able to phosphorylate a general kinase substrate, myelin basic protein, in vitro. The native substrate for SrkA has not been identified yet Zheng07.
  • DNA Length: 987 base pairs.
  • DNA sequence:

ATGAATAACA GCGCTTTTAC TTTCCAGACA CTACACCCGG ATACCATCAT GGACGCTCTG TTTGAGCATG GGATCCGGGT GGATTCCGGT CTTACCCCGC TTAACAGCTA TGAAAACCGT GTCTATCAAT TTCAGGACGA AGATCGTCGA CGTTTTGTCG TCAAATTTTA TCGCCCTGAA CGTTGGACAG CCGATCAAAT CCTCGAAGAA CATCAATTTG CGTTGCAGCT GGTAAATGAT GAAGTTCCGG TCGCAGCACC TGTGGCCTTT AACGGTCAGA CTTTATTGAA TCATCAGGGA TTTTATTTCG CTGTTTTTCC AAGCGTCGGT GGTCGCCAGT TCGAAGCTGA TAATATCGAT CAGATGGAAG CGGTTGGGCG TTATTTAGGG CGTATGCACC AGACGGGGCG CAAACAGCTT TTTATCCATC GCCCGACCAT CGGTTTGAAC GAATATCTCA TTGAGCCACG CAAGCTGTTT GAGGACGCTA CACTGATACC TTCCGGGTTG AAAGCGGCAT TCCTGAAAGC GACAGATGAG CTGATTGCCG CCGTTACAGC ACACTGGCGG GAAGATTTCA CCGTTCTGCG GCTACATGGA GACTGCCACG CCGGGAATAT TCTCTGGCGC GATGGTCCAA TGTTTGTTGA TCTGGATGAT GCACGTAATG GTCCAGCCGT TCAGGATTTG TGGATGTTGC TCAATGGCGA TAAAGCCGAG CAGCGGATGC AACTGGAAAC TATTATTGAA GCTTATGAAG AATTTAGCGA GTTCGACACC GCTGAAATCG GACTGATTGA ACCTTTACGC GCCATGCGTT TGGTTTATTA TCTTGCCTGG CTAATGCGGC GTTGGGCTGA TCCCGCGTTC CCGAAAAATT TCCCGTGGTT AACCGGGGAA GATTACTGGC TGCGACAGAC GGCGACTTTT ATAGAACAGG CAAAAGTTCT ACAAGAACCC CCTTTGCAAT TAACACCTAT GTATTAA

  • Amino Acid length: 328 amino acids.
  • Amino Acid sequence:

MNNSAFTFQT LHPDTIMDAL FEHGIRVDSG LTPLNSYENR VYQFQDEDRR RFVVKFYRPE RWTADQILEE HQFALQLVND EVPVAAPVAF NGQTLLNHQG FYFAVFPSVG GRQFEADNID QMEAVGRYLG RMHQTGRKQL FIHRPTIGLN EYLIEPRKLF EDATLIPSGL KAAFLKATDE LIAAVTAHWR EDFTVLRLHG DCHAGNILWR DGPMFVDLDD ARNGPAVQDL WMLLNGDKAE QRMQLETIIE AYEEFSEFDT AEIGLIEPLR AMRLVYYLAW LMRRWADPAF PKNFPWLTGE DYWLRQTATF IEQAKVLQEP PLQLTPMY

Function and Homologs

  • Product: stress response kinase A
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • Name: MULTISPECIES: stress response kinase A [Escherichia]/ Max score: 678/Query Cover: 100%/E-value: 0.0 /Ident: 99%/Accession: WP_001065496.1
    • Name: MULTISPECIES: stress response kinase A [Proteobacteria]/Max score: 678/Query Cover: 100%/E-value: 0.0/Ident: 100%/Accession: WP_001065497.1
    • Name: MULTISPECIES: stress response kinase A [Enterobacteriaceae]/Max score: 677/Query Cover: 100%/E-value: 0.0/Ident: 99%/Accession: WP_001765474.1

Expression

  • Expression Level: [Medium].
  • Expression Level Hypothesis: This enzyme phosphorylates the OH group of serine or threonine. Protein phosphorylation plays a significant role in a wide range of cellular processes and is a very important posttranslational modification, meaning it is probably relatively important to be abundant in the cell. The dataset shows that the expression level for for rdoA (related gene that also produces the protein Stress response kinase A) is medium, meaning that serine/threonine protein kinase is commonly used in this organism's proteins.
  • Expression Level References and Description: E. coli proteome data: File:EcoliProteomicExpressionData.xlsx
  • Expression Time: [Early].
  • Expression Level Hypothesis: Because protein phosphorylation plays a significant role in a wide range of cellular processes and is a very important posttranslational modification, it can be expressed at different times within an organism's lifecycle. Since this gene is involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion, cell development and transcription regulation, it is probably expressed early on.
  • Expression Time References and Description: Serine/threonine-specific protein kinase

Gene Context

  • Other Components in the functional module: Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline, needed. PLD1 encodes a phosphatidylcholine-specific phospholipase, necessary for protein phosphorylation.
  • Possible Dependencies: The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton
  • Process: Protein phosphorylation
    • Inputs: ATP, a protein
    • Outputs: ADP, a phosphoprotein

Construct

We will handle this - not part of your assignment

  • Synthesis Score: The synthesis score: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details:
  • GenBank File: A link to the GenBank file. file