EG12070

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Author Information

Jessica Hui

Basic Information

  • ID: EG12070
  • Name: yojl
  • Organism: E. coli
  • UniProt ID: [1]
  • Description: ABC transporters are conserved across all kingdoms, named for their soluble cytoplasmic ATPase subunits, also known as ATP-binding proteins or Traffic ATPases. Inner membrane translocation is facilitated by associated permease subunits or domains. Periplasmic binding proteins facilitate ABC uptake systems. ABC transporters import and export a variety of substrates in E. coli and humans, and include multidrug exporters and the diseased proteins of cystic firbrosis in humans. Import ABC transporters can include permease and periplasmic binding protein subunits; export ABC transporters can include permease proteins or domains that work with Outer membrane factor (OMF) proteins such as TolC and specific membrane-fusion proteins. Most ABC family ATP-binding proteins have been shown to be ATPases supplying energy to the ABC permeases of multicomponent ABC transporters, while some ABC family ATPases provide energy for non-transport processes. [1] [2]
  • DNA Length: 1644 base pairs.
  • DNA sequence:

atgGAACTTC TTGTACTTGT CTGGCGGCAG TATCGCTGGC CATTTATCAG TGTGATGGCG CTAAGCCTCG CCAGTGCGGC ATTAGGCATT GGCTTAATTG CTTTTATCAA TCAGCGCCTT ATCGAAACGG CGGATACCAG TCTGCTGGTG TTGCCGGAGT TTCTGGGATT ATTGCTGCTG TTGATGGCAG TCACTCTCGG ATCGCAACTG GCGCTCACCA CTTTGGGGCA TCACTTCGTT TACCGACTGC GTAGTGAATT TATCAAGCGG ATTCTGGATA CTCACGTCGA GCGCATTGAA CAACTCGGTA GCGCCTCGTT GCTGGCGGGG TTAACCAGCG ATGTGCGCAA TATCACCATT GCTTTTGTGC GTCTGCCGGA ACTGGTGCAG GGGATCATTC TCACTATCGG TTCAGCGGCG TATCTGTGGA TGCTGTCGGG CAAAATGTTG CTGGTAACGG CTATCTGGAT GGCGATCACC ATCTGGGGCG GTTTTGTGCT GGTGGCGCGG GTGTACAAAC ATATGGCGAC CCTGCGTGAA ACCGAAGACA AGCTGTACAC GGATTTTCAA ACTGTACTTG AAGGGCGCAA AGAGCTGACT CTGAACCGGG AACGCGCCGA GTATGTGTTT AACAACCTCT ACATTCCTGA TGCGCAAGAG TATCGCCACC ATATTATTCG CGCAGACACC TTCCATCTTA GTGCCGTGAA CTGGTCAAAC ATCATGATGC TGGGCGCAAT CGGCCTGGTG TTCTGGATGG CGAACAGCCT CGGTTGGGCT GATACCAACG TTGCCGCGAC CTATTCGTTG ACGCTTTTAT TCCTGCGTAC GCCGCTGCTT TCGGCGGTTG GCGCATTGCC GACGCTGCTG ACGGCGCAGG TGGCGTTTAA CAAGCTGAAC AAATTCGCGC TCGCGCCTTT CAAAGCAGAG TTTCCGCGCC CGCAGGCGTT TCCCAACTGG CAAACGCTGG AGCTGCGTAA CGTGACGTTT GCTTATCAGG ATAACGCGTT TTCCGTTGGT CCGATTAATC TCACCATCAA ACGTGGCGAG CTGCTGTTTC TGATTGGCGG CAACGGTAGC GGAAAATCGA CGCTGGCGAT GTTGTTGACG GGCTTGTATC AGCCACAAAG CGGCGAAATC TTGCTGGATG GCAAACCTGT CAGCGGCGAA CAACCGGAAG ATTATCGCAA ACTGTTTTCG GCAGTGTTTA CCGATGTCTG GCTGTTTGAT CAACTGCTGG GGCCGGAGGG TAAACCCGCT AACCCGCAAC TGGTTGAGAA GTGGCTGGCG CAGCTGAAAA TGGCTCATAA GCTTGAGTTA AGCAACGGGC GTATTGTTAA CCTGAAGTTA TCAAAAGGGC AGAAAAAACG CGTGGCGCTG TTGCTGGCGC TGGCAGAAGA ACGCGATATT ATCCTGCTGG ATGAATGGGC GGCGGATCAG GATCCACACT TCCGTCGTGA GTTTTATCAG GTGTTGCTGC CGCTGATGCA GGAGATGGGT AAAACTATTT TCGCTATCAG TCATGATGAT CATTACTTTA TCCACGCCGA CCGCCTGCTG GAAATGCGCA ATGGGCAACT TAGCGAGCTG ACGGGCGAAG AGCGCGATGC CGCTTCGCGT GATGCCGTTG CCCGGACGGC Ataa

  • Amino Acid length: 547 amino acids.
  • Amino Acid sequence:

MELLVLVWRQ YRWPFISVMA LSLASAALGI GLIAFINQRL IETADTSLLV LPEFLGLLLL LMAVTLGSQL ALTTLGHHFV YRLRSEFIKR ILDTHVERIE QLGSASLLAG LTSDVRNITI AFVRLPELVQ GIILTIGSAA YLWMLSGKML LVTAIWMAIT IWGGFVLVAR VYKHMATLRE TEDKLYTDFQ TVLEGRKELT LNRERAEYVF NNLYIPDAQE YRHHIIRADT FHLSAVNWSN IMMLGAIGLV FWMANSLGWA DTNVAATYSL TLLFLRTPLL SAVGALPTLL TAQVAFNKLN KFALAPFKAE FPRPQAFPNW QTLELRNVTF AYQDNAFSVG PINLTIKRGE LLFLIGGNGS GKSTLAMLLT GLYQPQSGEI LLDGKPVSGE QPEDYRKLFS AVFTDVWLFD QLLGPEGKPA NPQLVEKWLA QLKMAHKLEL SNGRIVNLKL SKGQKKRVAL LLALAEERDI ILLDEWAADQ DPHFRREFYQ VLLPLMQEMG KTIFAISHDD HYFIHADRLL EMRNGQLSEL TGEERDAASR DAVARTA

Function and Homologs

  • Product: ABC transporter ATP-binding protein
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • ABC transporter ATP-binding protein [Proteobacteria], 1108/100%/0.0/100%, [2]
    • ABC transporter ATP-binding protein [Enterobacteriaceae], 1107/100%/0.0/99%, [3]
    • ABC transporter ATP-binding protein [Escherichia], 1107/100%/0.0/99%, [4]

Expression

  • Expression Level: [low]
  • Expression Level Hypothesis: There have been hundreds of ABC transporters that have been characterized from both prokaryotes and eukaryotes. Thus expression levels for a single type of ABC transporter will be low because there are so many types.
  • Expression Level References and Description: M. genitalium model data, File:MgenitaliumSimProteinCounts.xlsx, Choi CH (Oct 2005). "ABC transporters as multidrug resistance mechanisms and the development of chemosensitizers for their reversal". Cancer Cell International. 5: 30. doi:10.1186/1475-2867-5-30. PMC 1277830 Freely accessible. PMID 16202168
  • Expression Time: [late]Since ABC transporters play a large role in transporting substances such as ions, amino acids, peptides, and sugars, this transportation is especially important later in an organism's life cycle, especially in cell growth and cell division. So, this gene will likely be expressed at later stages.
  • Expression Time References and Description: [[5]]

Gene Context

  • Other Components: ATPase, AAA family - MMSYN1_0132
  • Possible Dependencies: Periplasmic binding proteins facilitate ABC uptake systems. [3]
  • Process: Substrate transporting
    • Inputs: ions, amino acids, peptides, sugars from cytoplasm
    • Outputs: ions, amino acids, peptides, sugars to periplasm [4]

Construct

We will handle this - not part of your assignment

  • Synthesis Score: The synthesis score: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details:
  • GenBank File: A link to the GenBank file. file
  • http://www.ecogene.org/old/topic.php?topic_id=450
  • http://www.uniprot.org/uniprot/C7LMM4
  • http://www.ecogene.org/old/topic.php?topic_id=450
  • https://en.wikipedia.org/wiki/ATP-binding_cassette_transporter#ATP_binding_and_hydrolysis
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