EG12188

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Author Information

Alexander Akesson

Basic Information

  • ID: EG12188
  • Name: ptsP
  • Organism: E. coli
  • Description: EG12188 is the gene that codes for the phosphoenolpyruvate-protein phosphotransferase enzyme. This catalyzes the reaction between phosphoenolpyruvate and protein L-histidine to create pyruvate and protein N(pi)-phospho-L-histidine [1]. Specifically, the phosphoenolpyruvate-protein phosphotransferase enzyme only works on histidine residues in certain phosphocarrier proteins with low molecular masses which are taking part in bacterial sugar transport [2]. The phosphoenolpyruvate-protein phosphotransferase enzyme is part of the enzymes transferring phosphorus-containing groups combined with a nitrogenous group as an acceptor within the "transferases" family [3].
  • DNA Length: 2247 base pairs.
  • DNA sequence:

ATG CTT ACA CGT CTG CGC GAG ATC GTG GAA AAG GTA GCG AGT GCA CCC CGC CTT AAT GAG GCC CTG AAC ATC CTT GTA ACG GAT ATT TGC CTT GCA ATG GAC ACA GAG GTG TGT TCC GTA TAC CTT GCA GAC CAC GAT CGC CGC TGC TAT TAC CTT ATG GCC ACA CGT GGG CTG AAG AAA CCA CGT GGA CGT ACG GTA ACC TTA GCA TTC GAC GAA GGG ATT GTG GGT TTA GTG GGC CGC CTT GCG GAG CCC ATC AAT CTG GCC GAC GCT CAG AAG CAT CCA TCG TTT AAA TAC ATC CCC TCG GTA AAA GAG GAA CGC TTT CGT GCG TTC TTG GGA GTG CCT ATT ATT CAA CGT CGC CAG CTT CTT GGG GTA TTA GTA GTT CAG CAA CGC GAA CTT CGC CAG TAT GAT GAG TCG GAG GAG TCT TTT CTG GTG ACG TTG GCT ACT CAG ATG GCC GCC ATC CTG AGC CAG AGC CAA CTG ACG GCA CTT TTC GGC CAG TAC CGC CAA ACG CGC ATC CGT GCG TTG CCA GCC GCT CCA GGA GTA GCT ATC GCT GAA GGG TGG CAG GAT GCG ACA CTG CCG CTG ATG GAG CAA GTC TAC CAA GCA TCG ACC CTG GAC CCG GCA TTA GAG CGC GAG CGC TTA ACC GGT GCC CTT GAG GAG GCC GCA AAT GAA TTC CGT CGC TAC TCA AAG CGT TTC GCT GCG GGG GCT CAA AAG GAG ACC GCG GCG ATT TTT GAC TTA TAT TCT CAT CTT CTT TCC GAC ACA CGC CTG CGC CGC GAA TTG TTT GCC GAA GTA GAT AAG GGG TCA GTT GCG GAG TGG GCC GTC AAG ACG GTT ATT GAA AAA TTT GCT GAG CAA TTC GCT GCT CTT AGT GAC AAT TAC TTG AAG GAG CGT GCT GGA GAT CTG CGT GCG TTA GGG CAG CGT TTA CTG TTT CAT CTT GAC GAC GCA AAT CAA GGA CCA AAC GCG TGG CCG GAG CGT TTT ATC TTG GTT GCA GAC GAG CTT TCC GCC ACA ACC TTG GCT GAA CTG CCA CAA GAT CGC CTG GTG GGG GTG GTT GTT CGC GAT GGT GCG GCC AAT TCG CAC GCA GCC ATC ATG GTT CGT GCA CTT GGG ATT CCC ACT GTA ATG GGC GCT GAC ATC CAA CCT AGT GTG CTG CAC CGC CGC ACA TTA ATT GTC GAC GGT TAC CGT GGC GAA CTG TTA GTT GAC CCA GAA CCG GTT TTA TTG CAG GAA TAT CAG CGT TTA ATC TCC GAA GAG ATT GAA CTT TCG CGT TTA GCA GAA GAC GAT GTA AAC CTT CCC GCT CAG TTG AAG TCT GGT GAG CGT ATT AAA GTA ATG CTG AAT GCT GGT TTA TCC CCA GAA CAC GAG GAG AAA CTT GGT AGT CGT ATC GAC GGG ATT GGT TTA TAC CGT ACA GAA ATT CCC TTC ATG CTT CAG TCG GGC TTT CCA TCG GAA GAG GAG CAA GTT GCT CAA TAT CAA GGG ATG CTT CAG ATG TTT AAT GAC AAA CCA GTG ACG TTG CGC ACT CTT GAC GTT GGG GCG GAC AAG CAG CTT CCA TAC ATG CCC ATC TCC GAA GAG AAC CCG TGC CTT GGG TGG CGT GGA ATC CGT ATC ACT TTA GAC CAG CCA GAA ATC TTC TTG ATT CAG GTA CGC GCC ATG TTA CGT GCC AAT GCT GCC ACG GGT AAC TTG AAC ATC CTG CTG CCT ATG GTT ACC TCC TTA GAT GAG GTC GAT GAG GCT CGC CGT CTT ATC GAA CGC GCA GGG CGC GAA GTG GAA GAG ATG ATT GGC TAC GAG ATC CCT AAG CCT CGC ATC GGG ATC ATG CTT GAG GTA CCG AGC ATG GTA TTT ATG TTG CCG CAT TTG GCC AAA CGC GTG GAT TTT ATT AGT GTG GGT ACA AAT GAC TTA ACC CAA TAC ATT CTG GCA GTG GAC CGC AAC AAC ACA CGC GTA GCG AAC ATT TAC GAC TCC CTT CAT CCT GCT ATG CTT CGT GCC TTG GCC ATG ATT GCC CGT GAG GCC GAG ATC CAT GGA ATC GAC CTG CGT CTG TGC GGG GAA ATG GCG GGG GAT CCG ATG TGC GTA GCC ATC TTG ATT GGC CTT GGG TAC CGC CAC TTG AGT ATG AAT GGT CGT AGT GTG GCC CGC GCC AAG TAC CTG CTT CGC CGC ATC GAC TAC GCG GAA GCG GAG AAC TTG GCT CAA CGC TCT TTG GAG GCA CAG TTG GCC ACG GAG GTC CGT CAC CAG GTC GCC GCC TTC ATG GAG CGC CGC GGA ATG GGC GGT TTG ATT CGC GGC GGA CTT TAA

  • Amino Acid length: 748 amino acids.
  • Amino Acid sequence:

MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSVKEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLTALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEEAANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVIEKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATTLAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGELLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVDEARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQYILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILIGLGYRHLSMNGRSVARAKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGMGGLIRGGL

Function and Homologs

  • Product: phosphoenolpyruvate-protein phosphotransferase
  • Module: Phosphotransferase system (phosphoenolpyruvate-protein phosphotransferase is part of a larger system, PTS, which transports sugars into the cell).
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated [Escherichia coli], Max score: 1515, Query Cover: 100%, E-Value: 0.0, Ident: 99%, APE78620.1
    • phosphoenolpyruvate--protein phosphotransferase PtsP [Shigella sonnei], Max score: 1514, Query Cover: 100%, E-Value: 0.0, Ident: 99%, WP_077125817.1
    • phosphoenolpyruvate--protein phosphotransferase PtsP [Shigella flexneri], Max score: 1513, Query Cover: 100%, E-Value: 0.0, Ident: 99%, WP_025758805.1
  • Equivalent E. coli / JCVI functional protein: MMSYN1 0233.

Expression

  • Expression Level: medium
  • Expression Level Hypothesis: The ptsP gene is part of the the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). It is needed by the cell since it helps create pyruvate[4], which is needed for cell respiration.
  • Expression Level References and Description: The ptsP gene was in the E. coli proteome.
  • Expression Time: At which time should the gene be expressed in the lifecycle of our organism? Early
  • Expression Level Hypothesis: Cell respiration is essential for cellular growth, and pyruvate is an essential component. Thus it is essential to express this gene early.
  • Expression Time References and Description: Research description above and UniProt.

Gene Context

  • Other Components: ptsN ptsN, Phosphotransferase enzyme IIA component
  • Possible Dependencies: Phosphoenolpyruvate carboxylase Phosphoenolpyruvate carboxylase is needed so that phosphoenolpyruvate is created, which is needed for the PTS system to function [1][2]
  • Process: phosphoenolpyruvate-dependent sugar phosphotransferase system
    • Inputs: Phosphoenolpyruvate, protein L-histidine
    • Outputs: pyruvate, rotein N(pi)-phospho-L-histidine
    • Reference: [3]

Construct

  • Synthesis Score: The synthesis score of your construct: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details: For example, had to use a rare codon to fix folding energy;
  • GenBank File: A link to the GenBank file. file
  • https://ecocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=GO:0008965
  • http://www.brenda-enzymes.org/enzyme.php?ecno=2.7.3.9
  • https://en.wikipedia.org/wiki/Phosphoenolpyruvate%E2%80%94protein_phosphotransferase
  • http://www.uniprot.org/uniprot/P37177
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