EG12459 bennett7

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Author Information

Author Name

Basic Information

  • ID: EG12459
  • Name: AlsR
  • Organism: E.Coli
  • Description: "Allose utilization regulator," AlsR, negatively controls the expression of genes involved in transport and catabolism of D-allose and low-affinity transport of D-ribose [Sorensen96, Kim97]. It is negatively autoregulated and coordinately represses transcription of the divergent gene rpiB, encoding an enzyme in the pentose pathway [Sorensen96].
  • DNA Length: 891 base pairs.
  • DNA sequence:

atgAGCCAGT CAGAGTTTGA TTCAGCGCTT CCGAACGGTA TAGGGTTAGC GCCTTACCTG CGAATGAAGC AGGAAGGAAT GACAGAAAAT GAAAGCCGCA TCGTGGAGTG GTTACTCAAA CCCGGTAACC TGAGTTGTGC ACCCGCAATT AAAGATGTCG CAGAAGCTCT GGCGGTATCT GAAGCGATGA TAGTTAAGGT ATCAAAGCTG CTGGGGTTTA GCGGCTTTCG TAACTTACGC AGTGCGCTGG AAGATTATTT TTCTCAGTCA GAACAGGTAT TGCCTTCCGA GTTGGCTTTT GATGAAGCGC CGCAGGATGT GGTGAATAAG GTATTTAACA TCACTTTACG CACCATTATG GAAGGTCAGT CGATCGTCAA CGTTGATGAG ATCCACCGTG CCGCCCGCTT TTTCTATCAG GCCAGACAGC GGGATTTGTA CGGTGCCGGA GGATCAAATG CTATCTGTGC TGATGTACAG CACAAGTTCT TGCGCATTGG CGTACGCTGT CAGGCCTATC CTGATGCTCA CATCATGATG ATGTCCGCTT CGTTGTTACA GGAAGGAGAT GTTGTGCTGG TAGTGACCCA TTCCGGGCGA ACCAGTGATG TAAAAGCGGC CGTAGAACTG GCAAAAAAGA ACGGGGCAAA GATTATTTGT ATAACCCATA GCTACCATTC ACCGATAGCG AAACTGGCCG ATTATATTAT TTGCTCACCA GCCCCGGAAA CGCCGTTATT AGGTCGTAAT GCCTCGGCAA GAATATTACA ACTAACTTTG CTGGACGCTT TTTTTGTCTC TGTCGCCCAG CTCAACATTG AACAAGCTAA TATTAATATG CAAAAAACCG GCGCAATTGT TGATTTCTTC TCACCAGGCG CGCTGAAAta a

  • Amino Acid length: 297 amino acids.
  • Amino Acid sequence:

MSQSEFDSAL PNGIGLAPYL RMKQEGMTEN ESRIVEWLLK PGNLSCAPAI KDVAEALAVS EAMIVKVSKL LGFSGFRNLR SALEDYFSQS EQVLPSELAF DEAPQDVVNK VFNITLRTIM EGQSIVNVDE IHRAARFFYQ ARQRDLYGAG GSNAICADVQ HKFLRIGVRC QAYPDAHIMM MSASLLQEGD VVLVVTHSGR TSDVKAAVEL AKKNGAKIIC ITHSYHSPIA KLADYIICSP APETPLLGRN ASARILQLTL LDAFFVSVAQ LNIEQANINM QKTGAIVDFF SPGALK

Function and Homologs

  • Product: AlsR protein
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • Name, Max score/Query Cover/E-Value/Ident, [link Accession]
    • Name, Max score/Query Cover/E-Value/Ident, [link Accession]
    • Name, Max score/Query Cover/E-Value/Ident, [link Accession]
  • Equivalent E. coli / JCVI functional protein: A link to the identified E. coli functional analog of the protein encoded by the gene, or a link back to the JCVI gene matching this E. coli version. For example, MMSYN_0042 bennett7.

Expression

  • Expression Level: low
  • Expression Level Hypothesis: as a regulator, it would only expressed when called upon
  • Expression Level References and Description: [1]
  • Expression Time: impossible to tell
  • Expression Level Hypothesis: Not entirely sure of this one!
  • Expression Time References and Description: [2]

Gene Context

  • Other Components: No other components.
  • Possible Dependencies: no dependencies
  • Process: carbohydrate derivative metabolic process / Pentose Pathway!
    • Inputs: Glucose 6-phosphate, NADP+, H2O
    • Outputs: ribulose 5-phosphate, NADPH, H+, CO2 [3]

Construct

We will handle this - not part of your assignment

  • Synthesis Score: The synthesis score: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details:
  • GenBank File: A link to the GenBank file. file