EG50002

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Author Information

Gordon Dean

Basic Information

  • ID: EG50002
  • Name: rpmA
  • Organism: E. coli
  • Description: rpmA encodes the L27 protein, which is a component of the 50S subunit of the ribosome and may be an integral and functional component of the peptidyl transferase center [Hindennach71, Chen75]. Single-molecule fluorescence resonance energy transfer studies have indicated that L27 stabilizes the peptidyl-tRNA [Wang12d]. L27 participates both in the assembly of the 50S subunit and in the peptidyl transferase reaction [Wower98, Maguire05]. L27 crosslinks to aminoacylated tRNA in the A and P site [Wower89, Parker83, Rosen93, Osswald95, Maguire05]. Involvement of L27 with the peptidyl transferase center was also suggested by its interactions with various antibiotics: N-bromoacetyl chloramphenicol, an antibiotic that interacts with the peptidyl transferase center, affinity labels L27 [Sonenberg73]. L27 is labeled by the macrolides carbomycin A, niddamycin and tylosin, which inhibit ribosomal activity [Tejedor85]. L27 can also be crosslinked to the spiramycin derivative dihydrospiramycin [Bischof95]. L27 interacts with domain V of 23S rRNA [Osswald90, Urlaub95, Thiede98] and can be crosslinked to L33 [Redl89]. L27 is exposed on the surface of the 70S ribosome [Agafonov97].
  • DNA Length: 255 base pairs.
  • DNA sequence:

ATG GCC CAC AAG AAA GCG GGG GGA TCA ACT CGC AAC GGA CGC GAC TCT GAA GCG AAG CGC CTT GGA GTA AAA CGC TTC GGA GGT GAG AGT GTA TTG GCG GGC TCC ATC ATC GTG CGT CAA CGT GGC ACT AAA TTC CAC GCT GGC GCC AAC GTC GGA TGT GGG CGC GAC CAC ACA TTG TTT GCA AAG GCT GAC GGG AAG GTG AAA TTT GAA GTG AAA GGC CCC AAA AAT CGT AAA TTT ATT TCC ATT GAA GCA GAA

  • Amino Acid length: 85 amino acids.
  • Amino Acid sequence:

MAHKKAGGST RNGRDSEAKR LGVKRFGGES VLAGSIIVRQ RGTKFHAGAN VGCGRDHTLF AKADGKVKFE VKGPKNRKFI SIEAE

Function and Homologs

  • Product: 50S ribosomal protein L27
  • Closest homologous proteins:
    • 50S ribosomal protein L27 (Escherichia coli), 168/100%/4e-53/99%, WP_074494566.1
    • 50S ribosomal protein L27 (Escherichia coli), 168/100%/6e-53/99%, WP_069900792.1
    • 50S ribosomal protein L27 (Escherichia coli), 168/100%/7e-53/99%, WP_032151232.1
  • Equivalent E. coli / JCVI functional protein: MMSYN1 0499

Expression

  • Expression Level: high
  • Expression Level Hypothesis: rpmA, which is a part of a functioning ribosome, contributes to building all proteins in a cell, so more ribosomes (and thus more rpmA) will yield faster cell growth.
  • Expression Level References and Description: Annotated high in E. coli proteome
  • Expression Time: Early
  • Expression Level Hypothesis: Functioning ribosomes are necessary in order to produce new proteins, so we need to produce ribosomes early in order to synthesize later components.
  • Expression Time References and Description: Based on the gathered expression and function information

Gene Context

Construct

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  • Synthesis Score: The synthesis score: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details:
  • GenBank File: A link to the GenBank file. file