MMSYN1 0046 msnelson

From BioE80 Boot
Jump to: navigation, search

Author Information

Meredith Nelson SUID: msnelson

Basic Information

  • ID: MMSYN1_0046
  • Name: recR: recombination protein RecR
  • Organism: JCVI-Syn3.0
  • UniProt ID: A0A014MET1 [1]
  • Description: This is a homolog for RecR. RecR is a component of the RecFOR complex which functions in RecA-mediated replication recovery. Citation in [2]
  • DNA Length: 591 base pairs
  • DNA sequence:

ATG CTG GAG ACC ACT TTT GAT GAA ATC ATC GAA TCC ATT CGT ACT AAC CAG GGA TTG ACC AAA AAG ACC AGC GAG CGT CTT TTG GTG GAT CTG TTA ATC AAT AAG GAT AAG CTT GAT CAG TTT ATT GAC CAG TTG AAC AAG GCA AAG CAG CTG ATC TCA ACT TGC AAA ATC TGT GGC TAC TTA AGT GAA AAT GAT AAG TGC CTG GTA TGC TCG TTG GAG AAC CGC AAC CAG AAT ATC ATC TGT ATT GTA TCC ACC ATT CTG GAT GCT AAA AAC ATT GAG AAC ACC AAT AAG TAC AAG GGA GTG TAC CAC ATT CTT AAC GGT GAA ATT AAC TTA AAC AAA AAC ATC ACC CTT GAT AAG CTG AAT ATC TCG TCT ATC TTT AAG CGC ATC AAC GAT AAC ACT GAG ATT ATT TTG GCC CTG AAT AGC ACA TTC GAG GGG GAA CTT ACG GCC AAT TAC TTG TAT AAG CTT TTG TCA ACA AAG AAT ATC AAG ATT ACC CGC CTT GCA AAA GGG ATC CCA ATG GGT GCG TCC CTT GAC TAC ATG GAC GAA TTT ACA CTG CAA TCA GCG TTC CTG AAT CGT AAG AAG TAT GGG GAA TAA

  • Amino Acid length: 196 amino acids
  • Amino Acid sequence:

MLETTFDEIIESIRTNQGLTKKTSERLLVDLLINKDKLDQFIDQLNKAKQLISTCKICGYLSENDKCLVCSLENRNQNIICIVSTILDAKNIENTNKYKGVYHILNGEINLNKNITLDKLNISSIFKRINDNTEIILALNSTFEGELTANYLYKLLSTKNIKITRLAKGIPMGASLDYMDEFTLQSAFLNRKKYGE

Function and Homologs

  • Functional Category: DNA repair
  • Product: recombination and repair
  • Module: RECFOR
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • DNA repair protein RecM [Mycoplasma mycoides subsp. capri str. GM12], Max score = 387 / Query Cover = 100% / E-Value = 3e-136 / Ident = 100% [3]
    • recombination protein RecR [Mycoplasma mycoides], Max score = 386 / Query Cover = 100% / E-Value = 4e-136 / Ident = 100% [4]
    • Recombination protein [Mycoplasma mycoides subsp. capri PG3], Max score = 387 / Query Cover = 100% / E-Value = 1e-135 / Ident = 99% [5]
  • Equivalent E. coli: EG10834 [6]

Expression

  • Expression Level: Medium
  • Expression Level Hypothesis: The expression level for recombination is usually medium, and its close relative RecA also has a medium expression level. Additionally, the equivalent E coli homolog has a medium expression level
  • Expression Level References and Description: Citation for the expression level for its close relative RecA [7] The equivalent E coli homolog has a medium expression level, as determined by finding the uniprot ID of the E. coli for RecR in the spreadsheet cited and using the associated expression level [8]
  • Expression Time: Anytime during the organism lifecycle
  • Expression Level Hypothesis: Recombination must be able to occur at any point in the organism's lifecycle because issues can arise at anytime
  • Expression Time References and Description: Information about DNA recombination expression and timing is found at NCBI [9]

Gene Context

  • Other Components: RecA is a component, found in MMSYN1_0358 [10] The RecO DNA repair protein MMSYN1_0404 [11] also has RecA as a component, meaning it is a component of this gene as well
  • Possible Dependencies: amino acid biosynthesis, ATP synthesis, nucleotide synthesis, protein synthesis
  • Process: Recombination
    • Inputs: DNA template, enzyme that does that does recombination (ligase), free nucleotides, enzyme to seal the ends of the DNA, energy (ATP), cofactors (magnesium, other salts that help the enzymes work)
    • Outputs: Recombined DNA