MMSYN1 0129

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Author Information

Sharman Tan

Basic Information

  • ID: MMSYN1_0129
  • Name: PyrG
  • Organism: Mycoplasma mycoides​
  • Description: PyrG catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates. This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP [1].
  • DNA Length: 1599 base pairs.
  • DNA sequence:

ATG GCA AAA TTC ATT TTT GTC ACT GGT GGG GTG GTC TCA GGG TTA GGT AAA GGT ATT ACC GCT AGT TCT ATC GGG GCT TTG TTG AAG GCG AGC GGC TTA AAA GTC TTC ATG CAA AAG TTT GAT CCA TAC CTT AAC GTC GAC CCA GGA ACC ATG AGC CCT TAC CAA CAC GGG GAA GTA TTT GTT ACC AAG GAT GGA GGG GAA ACG GAT TTG GAT TTA GGA CAT TAC GAA CGT TTC ATC GAT GAA GAA CTT ACT AAA CTT AGT TCT ACC ACA TCC GGG AAG ATT TAT CTG TCT GTA ATT CAG GGT GAA CGC AAA GGG GTA AAT TCA GGG AAG ACC ATC CAA GTA GTG CCT CAT ATT ACC GAC GCC ATC AAA CAG AAG GTT TAC CAG GCG GCT GAA CAA TCT CAG GCA GAC GTC ATC ATC TCG GAA ATT GGG GGA ACA GTA GGC GAT ATT GAG AGT CAA CCC TTT ATC GAG GCG ATT CGC CAG ATT CGC TTA GAG CAG GGT AAA GAA AAC GTG ATG TTC GTT CAC GTT GTG TTG TTG TTA TGG TTA GCC GCT TCG AAA GAG TAT AAA ACT AAG CCC ATC CAG AAC TCC GTA AAG GCG ATG GCA TCT CTT GGG ATT CAG CCC GAT GTA ATT GTA TGC CGT AGT GAT AGC TCT TCT CCG AAG GAT ATT AAG GAG AAA ATT AGC CTG TTT TGT AAC GTG CCA ATT ACA AAC ATC ATC GAC GCG ATT GAC CAG GAT AGT ATC TAT CGT GTG CCG CTG GCC CTT GCC AAG CAG AAC ATT CAA GAC ATT ATC ATC GAG CAA CTT CAG CTG AAG GCC AAG AAC ATT GAT TTA TCT CTG TGG AAA CAA TTC AAC AAA AAG ATC GAT AGC TCA ACT GAG GAA ATT GAG ATC TCC TTT GTA GGC AAG TAC ATT GAA CTG CAA GAC GCG TAT CTT TCC GTG CTG GAG AGC CTG AAG ATC GCA GGG TGG GAG TTC AAC AAG AAA ATT AAA ATT CGC TGG GTG CAA GCT GAC AAA TTA GAC GAA TCA AAC TAC AAG GAA ATT TTG AAA AAC AGC CAA GGG ATT TTG GTA CCC GGC GGG TTT GGA AAG CGC GGT ATC GAG GGA ATG ATG TTA GCT TCT CGT TAT GCT CGC GAG AAT GAC ATT CCA TAT CTT GGG ATT TGC TTG GGC ATG CAG ATT GCA ACG ATT AGC ATT GCG CGT GAC CTG TTG AAT TGG ACA GAC GCC GAT AGT ACC GAA TTT AAC AAA AAC ACT ACT CAC CCA ATT TTT GAT TAC ATC AAA GGT ATT GAT CGT GAC AAC ATT GGA GGG ACG CTT CGC TTG GGC ACG ATG GTT ACA AAG CTT GAG AAG GAT AGC TTA GTC TCA AAA TTG TAT AAC AGC GAC GTT GCA TTA GAA CGC CAC CGT CAT CGT TAT GAG TTC AAC AAT GAG TAT AAG AAA GAT TTG GAG TCA GTT GGG TTA CGC TTT AGC GGG ATC TAC GAA GAG AAA AAC TTA GTA GAG GTA GTT GAA ATG CCC AGT TTA AAA TTT TTC GTC GCG AGC CAG TTT CAT CCT GAG TTT ACT TCT CGC CCG AAT AAA CCC ACT CCA CTT TTT CGT GGT TTT ATC AAA GCT ATT GTA GAG AAT AAC AAG TGA

  • Amino Acid length: 532 amino acids.
  • Amino Acid sequence:

MAKFIFVTGGVVSGLGKGITASSIGALLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVFVTKDGGETDLDLGHYERFIDEELTKLSSTTSGKIYLSVIQGERKGVNSGKTIQVVPHITDAIKQKVYQAAEQSQADVIISEIGGTVGDIESQPFIEAIRQIRLEQGKENVMFVHVVLLLWLAASKEYKTKPIQNSVKAMASLGIQPDVIVCRSDSSSPKDIKEKISLFCNVPITNIIDAIDQDSIYRVPLALAKQNIQDIIIEQLQLKAKNIDLSLWKQFNKKIDSSTEEIEISFVGKYIELQDAYLSVLESLKIAGWEFNKKIKIRWVQADKLDESNYKEILKNSQGILVPGGFGKRGIEGMMLASRYARENDIPYLGICLGMQIATISIARDLLNWTDADSTEFNKNTTHPIFDYIKGIDRDNIGGTLRLGTMVTKLEKDSLVSKLYNSDVALERHRHRYEFNNEYKKDLESVGLRFSGIYEEKNLVEVVEMPSLKFFVASQFHPEFTSRPNKPTPLFRGFIKAIVENNK

Function and Homologs

  • Product: CTP synthase
  • Module: NTP synthase
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • CTP synthetase [Mycoplasma mycoides subsp. mycoides], Max score: 1079, Query Cover: 100%, E-value: 0.0, Identity: 98%, AME13406.1
    • CTP synthetase [Mycoplasma leachii], Max score: 1059, Query Cover: 100%, E-value: 0.0, Identity: 96%, WP_013447496.1
    • CTP synthetase [Mycoplasma capricolum], Max score: 1055, Query Cover: 100%, E-value: 0.0, Identity: 96%, WP_036428457.1
  • Equivalent E. coli / JCVI functional protein: EG10810.

Expression

  • Expression Level: High
  • Expression Level Hypothesis: Because CTP is a necessary material in nucleotide synthesis, the expression level for pyrG is most likely high [2]. It is the rate-limiting enzyme for the synthesis of cytosine nucleotides from both the de novo and uridine salvage pathways [3]. Nucleotide synthesis is necessary for cells in the organism to perform DNA and RNA replication, which are necessary processes in order for genes to be expressed. Therefore, it makes sense that pyrG has a high expression level in the organism.
  • Expression Time: Right at the beginning
  • Expression Time Hypothesis: Because CTP is a necessary material in nucleotide synthesis, the expression level for pyrG is most likely high [4]. It is the rate-limiting enzyme for the synthesis of cytosine nucleotides from both the de novo and uridine salvage pathways [5]. Nucleotide synthesis is required for cells to perform RNA and DNA synthesis, which are necessary for the organism to express genes at the beginning of its life cycle. Therefore, it makes sense that the expression time for pyrG is right at the beginning of the organism's life cycle.

Gene Context

  • Other Components: GTPase
  • Possible Dependencies: ATP Synthase
  • Process: ATP-dependent amination of UTP to CTP
    • Inputs: UTP, L-glutamine, ammonia
    • Outputs: CTP
    • Reference: [6]

Construct

  • Synthesis Score: The synthesis score of your construct: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details: For example, had to use a rare codon to fix folding energy;
  • GenBank File: A link to the GenBank file. [7]