MMSYN1 0150

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Author Information

Eleanor Glockner

Basic Information

  • ID: MMSYN1_0150
  • Name: EF-G: translation elongation factor G
  • Organism: JCVI-Syn3.0
  • UniProt ID: F4MP38 [1]
  • Description: This protein catalyzes movement of tRNA from the A and P sites to the P and E sites during translation. [2]
  • DNA Length: 2070 bp
  • DNA sequence:

ATG GCG CGT GAG TAC TCT TTG CTG AAC ACA CGC AAC ATC GGA ATT ATG GCA CAC ATT GAT GCT GGT AAG ACA ACT ACA ACAGAG CGT ATT CTG TTT CAT ACG GGG AAG ATT CAT AAG ATT GGT GAA ACA CAC GAG GGG GCT TCC CAA ATG GAC TGG ATG GCACAA GAA CAA GAG CGT GGC ATC ACA ATT ACT AGT GCT GCG ACG ACT GCT TTT TGG AAA AAT ACA CGT TTC AAT ATT ATC GACACA CCA GGC CAC GTG GAT TTT ACT GTA GAG GTT GAG CGT TCA TTA CGT GTG TTA GAC GGA GCC GTT GCG GTC TTG GAC GGTCAA TCT GGT GTG GAA CCC CAG ACA GAA ACC GTG TGG CGT CAA GCT ACA AAT TAC AAA GTT CCT CGC ATT GTT TTT GTC AACAAA ATG GAC AAA ACA GGC GCG GAT TTC ATT TAC TCC GTC AAA ACG ATT GGA GAT CGT CTG GGG GCA AAA GCG GCT CCG ATTCAA TTA CCC ATC GGG GCT GAG GAG AAC TTC ACA GGC ATC ATT GAT CTT GTC GAA ATG AAG GCG TAC GAA TTT GAT GGA AAGCCT GAG GAA AAT TAT AAG GAG ATT GAG ATT CCT ACA AAC CTT TTG GAA CAG GCT AAG GAA TTG CGC GCA CAC CTT GTA GAAGTT GCT GTG GAA TAC GAT GAA GAA CTT CTG ATG AAA TTT CTT GAT GGT GGG GAA ATT TCC ATC TCA GAG CTT AAG TCA GCAATC CGT AAA GGT GTC ATC AAC GCG GAT TTC TTC CCC GTA TTG GCC GGA TCC GCC TTT AAG AAC AAA GGC GTA AAG TTG CTTTTG GAC GCG GTG GTA GAT TAT TTA CCA TCC CCC TTA GAC ATT CCC TCT ATT AAA GGT ATT TTA CCA ACT GGG GAG GAA GTAGAG CGC CAC GCG GAC GAT ACT GAG CCG TTT TCT GCA TTG GCC TTT AAG GTA ATG ACA GAC CCA TTT GTA GGA AAG TTA ACCTTC TTC CGC GTG TAT TCC GGT ATC CTG ACT AAG GGC TCG TAT GTG TTG AAC TCT ACT AAG CAA CAG AAG GAG CGT GTC GGTCGC ATT TTG CAG ATG CAT GCG AAT AAT CGT ACG GAA ATC GAA GAG GTG TAT AGC GGC GAC ATT GCC GCA GCC GTA GGC TTGAAA AAT ACC ACT ACG GGG GAT ACC TTA TGC GAC GAA AAG GGC GAA ATT ATC CTT GAA TCT ATG GTT TTC CCA GAG CCA GTGATC CAA TTG GCG TTA GAG CCC AAG ACG AAG GCC GAC CAA GAA AAA ATG AGT ATC GCG TTA TCA AAA CTG GCT GAG GAG GATCCG ACA TTC CGT ACA TAC ACA GAC GAT GAG ACG GGC CAG ACA ATT ATC GCT GGT ATG GGG GAA CTT CAT TTA GAT ATC ATTGTC GAC CGT ATG AAG CGT GAA TTC AAC GTA GCT ACG AAC GTA GGG GCG CCA CAG GTT AGT TAC CGC GAA ACC ATC AAG TTGCCG GGC AAG GCC GAG GGC AAA TAT ATC AAA CAA TCT GGA GGT CGC GGC TCC TAC GGG CAC GTG GTT ATC GAA TTC GAG CCGAAT AAA GAT AAG GGC TTT GAG TGG GTT GAT AAG ATT ACG GGC GGA CGC GTC AGT AAG GAG TAT ATC AAC AGT GCC CGT GTAGGA TTG GAA AAC GCC TTG CGC AAC GGG GTT ATC GCT GGG TAC CCG ATG ATC GAT GTT AAG GCA ACG ATT GTG GAC GGT TCCATG CAT GAA GTA GAT TCA AAC GAA ATG GCG TAC AAA ATC GCG GCA TCT ATG GCT CTT AAG GAA GCC TCC AAA AAA ATG AACCCT GTG GTG CTG GAG CCC ATC ATG AAC GTT GAG GTC ACG GTC CCT GAT GAG TAC TAC GGA GAC GTA ATG GGG AAC ATC TCTTCT AAA CGC GGC ATC ATT GAA GGA AGC GAA CAG CGC GGT AAT GCA CAG ACC ATC AAA AGT AAA GTG CCC TTA ACG GAA ATGTTC GGT TAT GCC ACT GAG CTG CGC TCC TTC ACG CAG GGA CGT GGG AAC TAC ACT ATG ATC TTC TCT CAC TAC GCT GAA GCCCCA AAG GCA ATC GCT GAT GAA ATC ATT AAG AAA TCA GGA AAA TAG

  • Amino Acid length: 689
  • Amino Acid sequence:

MAREYSLLNTRNIGIMAHIDAGKTTTTERILFHTGKIHKIGETHEGASQMDWMAQEQERGITITSAATTAFWKNTRFNIIDTPGHVDFTVEVERSLRVLDGAVAVLDGQSGVEPQTETVWRQATNYKVPRIVFVNKMDKTGADFIYSVKTIGDRLGAKAAPIQLPIGAEENFTGIIDLVEMKAYEFDGKPEENYKEIEIPTNLLEQAKELRAHLVEVAVEYDEELLMKFLDGGEISISELKSAIRKGVINADFFPVLAGSAFKNKGVKLLLDAVVDYLPSPLDIPSIKGILPTGEEVERHADDTEPFSALAFKVMTDPFVGKLTFFRVYSGILTKGSYVLNSTKQQKERVGRILQMHANNRTEIEEVYSGDIAAAVGLKNTTTGDTLCDEKGEIILESMVFPEPVIQLALEPKTKADQEKMSIALSKLAEEDPTFRTYTDDETGQTIIAGMGELHLDIIVDRMKREFNVATNVGAPQVSYRETIKLPGKAEGKYIKQSGGRGSYGHVVIEFEPNKDKGFEWVDKITGGRVSKEYINSARVGLENALRNGVIAGYPMIDVKATIVDGSMHEVDSNEMAYKIAASMALKEASKKMNPVVLEPIMNVEVTVPDEYYGDVMGNISSKRGIIEGSEQRGNAQTIKSKVPLTEMFGYATELRSFTQGRGNYTMIFSHYAEAPKAIADEIIKKSGK

Function and Homologs

  • Product: Elongation factor G
  • Closest homologous proteins:
    • Chain Y (bacterial ribosome), 882/98%/0.0/63%, [3J18_Y]
    • Chain B (70s ribosome), 880/98%/0.0/63%, [1JQM_B]
    • Elongation factor G (E. coli), 846/100%/0.0/60%, [WP_000124704.1]
  • Equivalent E. coli functional protein: EG10360

Expression

  • Expression Level: low
  • Expression Level Hypothesis: From the Mycoplasma genitalium simulation protein counts, we know that a cell has many different translation elongation factors, some expressed at high levels and some at low levels. It is hard to know why this particular elongation factor has a low expression level, other than there are many other elongation factors so it does not need to be high.
  • Expression Level References and Description: Mycoplasma genitalium simulation protein counts [3]
  • Expression Time: early
  • Expression Level Hypothesis: Because this gene helps facilitate translation, it is important for it to be expressed early to aid in biosynthesis of essential proteins.
  • Expression Time References and Description: Uniprot [4]


Gene Context

  • Other Components: ribosome, tRNA
  • Possible Dependencies: GTP
  • Process: translation
    • Inputs: mRNA, tRNA
    • Outputs: proteins coded by mRNA