MMSYN1 0371

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Author Information

Jessica Hui

Basic Information

  • ID: MMSYN1_0371
  • Name: ABC transporter, ATP-binding protein
  • Organism: JCVI-Syn3.0
  • UniProt ID: [1]
  • Description: ABC transporters are conserved across all kingdoms, named for their soluble cytoplasmic ATPase subunits, also known as ATP-binding proteins or Traffic ATPases. Inner membrane translocation is facilitated by associated permease subunits or domains. Periplasmic binding proteins facilitate ABC uptake systems. ABC transporters import and export a variety of substrates in E. coli and humans, and include multidrug exporters and the diseased proteins of cystic firbrosis in humans. Import ABC transporters can include permease and periplasmic binding protein subunits; export ABC transporters can include permease proteins or domains that work with Outer membrane factor (OMF) proteins such as TolC and specific membrane-fusion proteins. Most ABC family ATP-binding proteins have been shown to be ATPases supplying energy to the ABC permeases of multicomponent ABC transporters, while some ABC family ATPases provide energy for non-transport processes. [1] [2]
  • DNA Length: 1872 base pairs.
  • DNA sequence:

ATG AAA GTA AAG AAT AAT TTT GAT CAT TTT TAC AAG CCA ATG ACG GAC GAA GAG ATC AAG GCC GAC CGT AAG TCT TTT AAC CGT GGC CGT AAG TCC TTT ATC AAT GTT ATC TGG AAG CAC ATG AAG ATT AAC AAA AAG TGG GCC ATT GGA CTG TTA ATC ACG GCG ATC TTC TCC GCG TTA TTT GCA GCT TTG AAC CCC TTG CTG ATG CAA CAA CTG CAG TTT GCA GTC GAA TTT GAA AAA ACC CAC CAA AAC TTC TCG AAC AGT TGG GGG CTT TCT TGG AAA GTT ATC TTG GCA ATC TGG ATT GTG ATT TTA GTC ATT ACC GCA ATC TTA ACG TAT ATT GCG AAC TTA TTC GGA AAT GAA TTG GGG AAG AAA ATC GAA ATT AGC TTG CGC AAT GAG TTG ACA CGT AAA TTG ATT ACT ACG GAT ATC CAC TAT TAT TCT AAC AAG AAG ACA GGA GAA ATC TTG ACC AAA GTT GTA TCA GAT ACA CAG ATC ATC GGA ATG CAG GCC TCG GTT ATC CCT AAT ATT ATT TTC ACG GCC TTC TTT ACA ATG GTG TTC ACG CTT ATT ACA CTT TTC ATC ACA ACC TCG TTG TAC ATC GGG TTA TTC TTT ATC AGT TTA TTT TTA ATG TTT GGA ATT CTT TTT GGC CTG AGT TTC CTT CCG ATG CGT AAA TTG GTC TTC AAT CTT CGC AAG ATC ATT ACA GAC ATT AAT GGG GAC GTA ACA GAT CGC ATC AAC ACG ATC AAG TTG ATC AAG GCA AAC GGG ACT GAA GAG TAC GAG AAA ACA CGT TTT GTA CAA ATT CAC GAT GTT TAC TAT AAA AAG TAC AAA CAA ATC AGC TAT TTT CAA TCT GTT ATG ATC AGC ATC CTG TTT TTT GCG ATT AAC ACT GTA CAG ATT CTG ATG ACA TTG ATT GCC TTG TGG TTA TAC AAA AAT GAC ATC ACT ACG TTA AAA ACC ATT CTG GGT CCA ATG TTA ATC TGC GCT GGA ATG CTT ATC GGC CCA ATT ATG CAG TTG TTG CGC GCT ATC ATT GGC ATG GTT CAG GCA TCA ACC TCT GCC CAA CGT ATC GAC GAA ATC ACG GAC GCC ACT CAA TTA ATT AAT AAT CAT TCG TTG GAT AAG AAG GGG ATC CGT ATT CAC AAG ATC GAA GGT AAC TTA GTT TTT AAG AAC GTA AAT TTC TCA TAT CCC GAC AAA CCA GAG AAC GTC ATC CTG CCT AAT TTC AAT TTG GTA TTA GAA AAG GGA AAG AGT TAT GCG TTT GTT GGG CAA ACC GGA GCC GGA AAG TCT ACT ATC TCG AAA CTT TTA TTG CGC TTT TAT GAC CCT ACT AGT GGA GAA GTC TTG ATC AAC GAT AAT ATC AAT ATC AAA GAT GTT TTC CTG CCC TCT TAT TTA AAC CAC ATC GGG TAT GTT GAA CAA GAT CCC TCG GTA CTT TTA GGG ACA GTA TTC GAC AAC CTG CGC TAT GTC AAG CCA TCG GCG ACG GAC GAA GAA ATT ATT CTG GCT TGC AAG AAA GCC GAA TTG CAT GAC TTA GTA ACG ACT TGG CCA GAA CAG TAC AAT ACC ATT CTT GGG GAA CGT GGT TTC ATT TTG TCA GGC GGA CAA AAA CAG CGC CTG GTG ATC GCG CGT ATG TTT CTG AAG AAC CCA GAC ATC TTG ATT TTA GAT GAA GCT ACA TCA GCA CTT GAC AAC GTG GTC GAA AAA GAA ATC CAA GCC AAA TTA GAG GAA TTG ATG CAG GGG CGT ACC TCG ATC ACC ATC GCT CAT CGT TTA TCA ACC ATT CGC AAC GTT GAC CAA ATT ATT GTC TTG GCT CCA AAG AAA GGA ATT ATT CAG ATC GGG ACT TTT AAG GAA TTA GTG AAA AAG CCC GGC GAG TTC AAA GAT CTG TAT GAG GCC GGT TTC AGC AAA TAT GAT GCC TAG

  • Amino Acid length: 623 amino acids.
  • Amino Acid sequence:

MKVKNNFDHFYKPMTDEEIKADRKSFNRGRKSFINVIWKHMKINKKWAIGLLITAIFSALFAALNPLLMQQLQFAVEFEKTHQNFSNSWGLSWKVILAIWIVILVITAILTYIANLFGNELGKKIEISLRNELTRKLITTDIHYYSNKKTGEILTKVVSDTQIIGMQASVIPNIIFTAFFTMVFTLITLFITTSLYIGLFFISLFLMFGILFGLSFLPMRKLVFNLRKIITDINGDVTDRINTIKLIKANGTEEYEKTRFVQIHDVYYKKYKQISYFQSVMISILFFAINTVQILMTLIALWLYKNDITTLKTILGPMLICAGMLIGPIMQLLRAIIGMVQASTSAQRIDEITDATQLINNHSLDKKGIRIHKIEGNLVFKNVNFSYPDKPENVILPNFNLVLEKGKSYAFVGQTGAGKSTISKLLLRFYDPTSGEVLINDNINIKDVFLPSYLNHIGYVEQDPSVLLGTVFDNLRYVKPSATDEEIILACKKAELHDLVTTWPEQYNTILGERGFILSGGQKQRLVIARMFLKNPDILILDEATSALDNVVEKEIQAKLEELMQGRTSITIAHRLSTIRNVDQIIVLAPKKGIIQIGTFKELVKKPGEFKDLYEAGFSKYDA

Function and Homologs

  • Product: ABC transporter, ATP-binding protein
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • ABC transporter ATP-binding protein [Mycoplasma feriruminatoris], 1201/100%/0.0/94%, [2]
    • ABC transporter ATP-binding protein [Mycoplasma capricolum], 1146/99%/0.0/91%, [3]
    • ABC transporter ATP-binding protein [Mycoplasma leachii], 1144/99%/0.0/91%, [4]

Expression

  • Expression Level: [low]
  • Expression Level Hypothesis: There have been hundreds of ABC transporters that have been characterized from both prokaryotes and eukaryotes. Thus expression levels for a single type of ABC transporter will be low because there are so many types.
  • Expression Level References and Description: M. genitalium model data, File:MgenitaliumSimProteinCounts.xlsx, Choi CH (Oct 2005). "ABC transporters as multidrug resistance mechanisms and the development of chemosensitizers for their reversal". Cancer Cell International. 5: 30. doi:10.1186/1475-2867-5-30. PMC 1277830 Freely accessible. PMID 16202168
  • Expression Time: [late]
  • Expression Level Hypothesis: Since ABC transporters play a large role in transporting substances such as ions, amino acids, peptides, and sugars, this transportation is especially important later in an organism's life cycle, especially in cell growth and cell division. So, this gene will likely be expressed at later stages.
  • Expression Time References and Description: [[5]]

Gene Context

  • Other Components: ATPase, AAA family - MMSYN1_0132
  • Possible Dependencies: Periplasmic binding proteins facilitate ABC uptake systems. [3]
  • Process: Substrate transporting
    • Inputs: ions, amino acids, peptides, sugars from cytoplasm
    • Outputs: ions, amino acids, peptides, sugars to periplasm [4]


Construct

We will handle this - not part of your assignment

  • Synthesis Score: The synthesis score: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details:
  • GenBank File: A link to the GenBank file. file
  • http://www.ecogene.org/old/topic.php?topic_id=450
  • http://www.uniprot.org/uniprot/C7LMM4
  • http://www.ecogene.org/old/topic.php?topic_id=450
  • https://en.wikipedia.org/wiki/ATP-binding_cassette_transporter#ATP_binding_and_hydrolysis
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