MMSYN1 0399

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Author Information

Danny Pantuso

Basic Information

  • ID: MMSYN1_0399
  • Name: efflux ABC transporter, permease protein
  • Organism: JCVI-Syn3.0
  • UniProt ID: [1]
  • Description: The efflux ABC transporter is the disposal system protein for the cell. (Efflux means to throw out of, particularly a cell.)The permeases are membrane transport proteins, a class of multipass transmembrane proteins that facilitate the diffusion of a specific molecule in or out of the cell by using proton motive force, an active transport. [2] ABC transporters often consist of multiple subunits, one or two of which are transmembrane proteins and one or two of which are membrane-associated ATPases. The ATPase subunits utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to energize the translocation of various substrates across membranes, either for uptake or for export of the substrate.[3]
  • DNA Length: 5262 base pairs.
  • DNA sequence:

ATG AAA CGC ATT ACC TCG TTT CTG TTG CTT CTT AAA CAG GGC TTG AAG GGT GTG TTC AAG TTC AAA ATT CAA TTT ATT ATT ATC TTA TTG TTA TCT TTT TTG GCT TCG TTT ATT TTG TCG ACA AGT CTG ACT TTG ACC AGC CGT ATC AAT AAA ACC TAT AAC AAT ATT GTA AAC AAT GTA AAT AAA TTC GAT TAT TCG AGT ACT AAC GAA ATC CGC ACT TAC CGT ATT GAT CGT AAC AAC TCG ACA ACG GAC CGC TCG GTT ATT GCA TTA TTA GAC CTG GTA AAC AAC TCT AAC TCG TAT TAC AAC CAG TCG TCG AAT AAT AAG AAC ACT TCG TAT TTG AAT TTC ATT CTG AAC AAG AAA AAT TTA ACC TCT AAT TTC GAT AAT AAA ACC ATT CTG ACG GAG TTA TTC GAA AAC AAA GAG TTC ATC GAG CTT TTT ACC ACG ATT AAC GGC AAA GAC ACG AAC TGG ATT TGG GAG AAC ATC TGG CTG TGG CAG CTG AGC CTT TAC TTT AAT AAG TTC ATC TAC CAC TCA TAT GAT CAA TTC TTA AAG AAC AAC AAG GAC TAC TCA TAT CTG AAA AAT ACT GTC ATT GGT AAA TAC TTG TCG AAC TCA TTT AAA GAT AAA AAT GAG TTT TTA AAT GAT GCG AAA GTG TTG GAG AAT TTG AAA TTT GAA AAT ATT AAG AAT AAC TTT AAT GTG AAA GAG TTT AAA AAT ACA TTC AAT AAA CAG ATT CAG AAT AAG GAA CTG TTC TCG TAT ATC TAC ATT AGC GGT ATG AGT CTT TTC CAA CAT ATT TAT CGC AAC ATT TAT CTT CCA TAC TTT TCC GAC TTC AAA ATC ACG AAT AAT AAC AAG ATT GGG AAT AGC TTT TAC ACT TTT TTG ACC GGC AAT AAG CTT AAC AAC ATC AAT GAC AGC CAG GCG GAC AAA TGG ATC ATT AAT GAC AAA AAC AAA TCT TAT CTG ACC GAA TTT GAG CTG AAC AAG ACC ACG ATT GAT AAA AAC GAT AAT TCA GTA CTT ATT AAA ACC GAA TCT AAA GAC GAC ATC AAA AAA CTG GTG CTG GAA AAG GGG TTT AAG GGG AAT ACT GAT CTT GTT CTG TCA ACT ATT GAC AGC AAC AAC AAA GTA CAG TCC ATC AGT CCA ATC ATC AAT GAT AGC TCG TTC TTT AAG CTG CTG TTC TTT AAC GGC AAC GGG ACG TCC TTG ACA AAC GTG GTG ACG GTA CTG TCT GAT ATT AAT TTT ATC AAA AAG GAC CAA ATT ATC GGC GAA AAC CAA TTT GAC AAT ATC AAT TTA TTT CAT AAT ATC TGG TTG GCA CAC TTG AAA TAT ACC GCG ATC GCC TCG GGC TAC GAT ATT AAC TTC CGT ACG GAG GTT TTC AAC TAT GAT TCT GTT ACC CAG ATC CGC TAT CGC TTG GTC ATT TTA AAC GAT GAC CAT ACA ACT AAT CTT ACG ATC TTG AAC AAA AAT CAA GGC GCG CGC AGC CCG TCA AAA GGT GAG GCT TTG ATC TCG GAG CAG TTT GCC CGC GCA CAT AAG TTG AAG TTA GGG CAA CAG ATT ATT GTA GAC GGT GCG TTA CTG ACA ATT ACT GGG TTT GCG ACT GAC ACC TAT TCC TTT TTC CCT ACC ACC GAT CCT GAT TTC CCA ATT CCG CAG TCA GAA CTG GGC GCG ATT TTA TAC GTA ACT CGC TCG ACT ATT AAT GAT ATC TTG GGA GCG ACA AGT CAG TCT AAT ACT AAT CGT GTA AGT AAG GGA TAT TTA AGT TTC TTT TTG CGT AAA CGT CAG TCA AAC GCG AGC ATT AAT CTT TTT AAC TCC TAC CAA ATG AAT GAC ATT TCA AAA TTG TAT GAC TCG ATT AAG TAT CAG AAG GAC CAA AAA AAT AAG GTT ACT ACC TGG TTA AAT ATT AAG GAT TTC GAC CAC AGT ATT TTT CGT TTT AAT TGG ACG ATC GCC CCA CTG GCG ATC AAT AGC TAC AAG GGC GCA ACC TTA ATC GCA GCA CTT GTA GTT AGC TTG ATT GCG ATC ATT GCT CTT GTG ATT TGT ATT CGC AAA ACA ATC TAT TTC AAT GCT AAG CAG ATC GGC ATT CTT AAA GCC TTG GGA AGC TCG CCC ATC CAG ATC AGC ATT TCA TAC TTG GCG TAT GTA ATT GTG ATT ATC TTA ACT AGT GTA CCC CTT GGA TGG ATC ACC GGG CTT TCC ACT CAG AGT GTA TTC GTT AAG CTT TTT GTC AAT TAT TTT TCA ATC CCG CTT TAT TCG TTT ACT ATC GAA CCG TTT TCA CTT CTG ATT TCC CTG TTG ATC TTC GGG CTG TTT GGG GTC ATC GTA TCC CTG CTT TCT GCA ATC ATC ATC ACT AAG AAA CAG TTA GCC GAC ATT TTA GCA GTG AAA CAG AAT TGG TCT TCG TCT AAG TTC ATC AAT CGT TTA AAG CGT ACA TGG TTT AAA AAG GCT AAA TTT ACT ACC AAG TTT TCG TTA ACC CTG GCG TCG TCA GGT AAG AAA AAT ATT TTC CTT CTG GTG ACC GTA GTA GGA ATC TCC ACT ATG TTT ATT AGC GCC GGC CTT GCT ATT CCC AGC ATC GCG TTT ACT ATC AAG AAT ACG TAT TAT AAG TCT ATC AAG TAC GCC AAC GAG TAT AAC TAT TCA AAG GGT GTA TCC AAC AGC CCT TTG ACT AAG CCA ACG ATT AAT TAC TGG TCG GGC CAA GAC TCT CTG GAT AAA AAC ATT TTG TCT GCA AAC CTG AAT AAT GAA GAA CTG TTC TAC TAC AAG GAC CCT ACA GCG TAT GCC TCT TCC AGT TAC GAT GTA AAC CCA TTC CCC AAA TAC TTA TAT AAA GTC GAG AAG TTC AAT AAT AAC AAT AAT GAG CAG ATC AAC AAG AAA ATT GCT TGG ACC TTA CTT GAA TTG ATC CAG AAC AAA GAC CAA ACA AGC GCC AAT CAT ACG AAT GGT TTG GAT CTT TTG TTT ACA GAA ATG TTT GGT AAT AAC TTG TAT AAT GTT GTC GGA AAC CAG TTC TCG ATC GGC GTT ATC GAC CAA ATC CTT GGA TTA ATC CTT AAC TCT AAA AAT AAC GTT GTT AAT CCC AAG GAC ACG ACA ACG AAA TGG ACA GAC GAG CAG AAA GAT TTG ATC TTT AAA GAG TTG ACC AAC AAT TTT ACG AAG ACC GGA ACC ACA GCG ATC TCC ATT TTA GTC GGT GAC CTG AGT ACC TCT TCC TCC GAT GAT TGG AAG ACC AAG ATC TTT GAC GCA ATT TTG AAA GCG GTG CCG CCG TAC GTG AGC GCC TAC ATT CAG AAA CCC TCG CGC AAA GAA CAA TTT TCA ATC GGC TAC AAC GTT CAA CAT TAT ATT CCT GAT CAC GAG ACG TTA ACA ACA ATT ACG GAC ATC AAA ACG ACA ATT AAT CAG AAA AAC ACT GAT CTG TCG CTG ACT GGC ATT GCT AAT AAC CAA TCT GCC TTT ATT ATT AAC CAA AAG AAC GCC AAC AAC TTG TTC ATT GAC TAC AAG AAG TTA CTG GCG TTG CAG GAA GTA TTC TTG GAA AAG AAG AAC ACC GAT ATT AAA CTT AAT GAT CAG TTT GTT CTT TAT GAT TCC AAG ACG AAC ACT ATT AAC GTG CCC ATC TTG CCA AAC AAA CAG GCA AAC GCT TTC TAC AAG CTG AAT AAG AAC CCA GAT ATC TCG AAT ATC TCA ACG AGC TCG AAA CAG TTT TTT ATC AAT ACG AAA AAC GGT TAT GTC AAC ATT CCC AAA CAT GCG TGG ATC TAC GAT GAC CTG AAC TTC ATT AAG TCC AAG TAT TAT AAC AGC CTG ACC TCC GAA CAG AAG AAC TTG ATT TCG AAG AAT CGT ACT GGT CGC AAC TCG AAG ACG GTT AGC GAT CAG GAT ATC CGC TGG TTA GAC CCC TAC AAT TTA GAC AAT AAT AAA TTT ACC TTA AAA CTG TTG TAT GAT AAT GAC AAA TTT GAC AAC GAC TCT TCT TAT GAC AAT AAA GAA TGG TCT CTG TTG AAC AAT TCG TAC ATG TTC GAC GAT TTT ATC TAC AAC AAC CAA TTC GAT GAC TTA CTG TCC TCC TAC ATT CGT CCA TAC TAC CAA TAT AAG AAT ATC CAG TTA TAT ATC CCC CAG TCG TTG ATC AAC ACA GAT CAC ATC ATT CAC TTT ATC AGT TCT AAG AAA ACT AAA AAG GAA CTG GAC AAC AGC AGC GAG CAC TGG TAT AAG AAG GAC ATT GAT TAT AAT AAC GTT CCG AAA AGC GTA ATC AAG GCC TGG GAT ATT AAA AAT ACA TCC GAA AAG TTT TTG ATG ATT CGC CCT TAT GAT TTG CGC TAT TCA TTG CTG GTC GAT AAT GTG TAT AAA TCG GGC CTG TCG AAT CTT ACT GCA AAA CCG GAG TAC TGG ATG TAT CAA GCG ACA AAA ACT AAG AAT ATT TCG GGT ATT ACT ACA CCA ATT ATT CAA AAA GAC GCC AAA ACT AAC TAC CAA AAT AAG GAT TTG AAG ATC ACT ATT AAA CCC GTA GGG ACC TTG GAT TCT TAT AAT CAA AAG TTA ATC CTG GCG GAT CAA GGC CTG ATT AAT CTG GTA CTG AAT CTT TCG ATT GGA AAA AAG ATC GGA ATC AAG GAT AAC TTC TAC AAC AAG CAG ACA GTC ATT AAA GCG GGA GAA TCA TAC AAT AAT ATC ATT TCT CGC TTC GAC CGT TAT GAT TAT AAC CAG ATT ATC AAT TAT ATT GAC AAA ACG AAG AAT ACA AAA GAA TTC AAC GAC TTG TTA TTT AGT AGC AAT AAG GCT TTT GAC AAG GCG CAG TTT TTA TGG CAC AAT GCC AAA TAT TCA AAT ATC GAG GAA GCT CTT GAT TTA ACA TCT GGT ATC TCA TTT ATT CCA GAC ACC GCT TAC AAC GGT TTT TAT ATT CTT AAC GGT CAC GGA GCA TCT TCA GCC TCA GGA GAC GAC GAT ATG ATC AGT AAC ATC AAG AAC CAA AAC TTA CTT GCA ACC TCA AAA ACC TTG ATT AAT CAA ATT ACT TTC ATC GCG ATT TCA ATT GGC ATG TTA CTT ATC ATC ACC GTT ATC ATT ACT TCG GCG CTG CTG GTA ATG CTT ATT TCA GAC ATT TAC GTT ACA CAG TAT CAG CAA TTC ATG ATC TTA ATG AAA GCA CTT GGG TAC TCG AAC TAT AAA ATT TCT AAG TAC GCT TTT GGG ACC GCG ATC GTG TTC TCC CTT ATT ATG TGG GCT ATC TCA ACA TTA GCT ACA TGG ATC TTA ATC ACA CTG ATT ATT CAA ATC ATT ACT TCG TTA GGT TTC GCT ATC CCA TAC GGC TTT GCA TTC TGG ACA CTG ATT GTC TCT TTC ATC ATC ATC GGT ATC TCC TTC ATT GGC TCA CTG ATC GTA TCA TCA AAT AAA ATT CGT ACG CAG AAG CCG GCA TCG TTA TTG ACC GTA AGC AAT GAG taa

  • Amino Acid length: 1753 amino acids.
  • Amino Acid sequence:

MKRITSFLLLLKQGLKGVFKFKIQFIIILLLSFLASFILSTSLTLTSRINKTYNNIVNNVNKFDYSSTNEIRTYRIDRNNSTTDRSVIALLDLVNNSNSYYNQSSNNKNTSYLNFILNKKNLTSNFDNKTILTELFENKEFIELFTTINGKDTNWIWENIWLWQLSLYFNKFIYHSYDQFLKNNKDYSYLKNTVIGKYLSNSFKDKNEFLNDAKVLENLKFENIKNNFNVKEFKNTFNKQIQNKELFSYIYISGMSLFQHIYRNIYLPYFSDFKITNNNKIGNSFYTFLTGNKLNNINDSQADKWIINDKNKSYLTEFELNKTTIDKNDNSVLIKTESKDDIKKLVLEKGFKGNTDLVLSTIDSNNKVQSISPIINDSSFFKLLFFNGNGTSLTNVVTVLSDINFIKKDQIIGENQFDNINLFHNIWLAHLKYTAIASGYDINFRTEVFNYDSVTQIRYRLVILNDDHTTNLTILNKNQGARSPSKGEALISEQFARAHKLKLGQQIIVDGALLTITGFATDTYSFFPTTDPDFPIPQSELGAILYVTRSTINDILGATSQSNTNRVSKGYLSFFLRKRQSNASINLFNSYQMNDISKLYDSIKYQKDQKNKVTTWLNIKDFDHSIFRFNWTIAPLAINSYKGATLIAALVVSLIAIIALVICIRKTIYFNAKQIGILKALGSSPIQISISYLAYVIVIILTSVPLGWITGLSTQSVFVKLFVNYFSIPLYSFTIEPFSLLISLLIFGLFGVIVSLLSAIIITKKQLADILAVKQNWSSSKFINRLKRTWFKKAKFTTKFSLTLASSGKKNIFLLVTVVGISTMFISAGLAIPSIAFTIKNTYYKSIKYANEYNYSKGVSNSPLTKPTINYWSGQDSLDKNILSANLNNEELFYYKDPTAYASSSYDVNPFPKYLYKVEKFNNNNNEQINKKIAWTLLELIQNKDQTSANHTNGLDLLFTEMFGNNLYNVVGNQFSIGVIDQILGLILNSKNNVVNPKDTTTKWTDEQKDLIFKELTNNFTKTGTTAISILVGDLSTSSSDDWKTKIFDAILKAVPPYVSAYIQKPSRKEQFSIGYNVQHYIPDHETLTTITDIKTTINQKNTDLSLTGIANNQSAFIINQKNANNLFIDYKKLLALQEVFLEKKNTDIKLNDQFVLYDSKTNTINVPILPNKQANAFYKLNKNPDISNISTSSKQFFINTKNGYVNIPKHAWIYDDLNFIKSKYYNSLTSEQKNLISKNRTGRNSKTVSDQDIRWLDPYNLDNNKFTLKLLYDNDKFDNDSSYDNKEWSLLNNSYMFDDFIYNNQFDDLLSSYIRPYYQYKNIQLYIPQSLINTDHIIHFISSKKTKKELDNSSEHWYKKDIDYNNVPKSVIKAWDIKNTSEKFLMIRPYDLRYSLLVDNVYKSGLSNLTAKPEYWMYQATKTKNISGITTPIIQKDAKTNYQNKDLKITIKPVGTLDSYNQKLILADQGLINLVLNLSIGKKIGIKDNFYNKQTVIKAGESYNNIISRFDRYDYNQIINYIDKTKNTKEFNDLLFSSNKAFDKAQFLWHNAKYSNIEEALDLTSGISFIPDTAYNGFYILNGHGASSASGDDDMISNIKNQNLLATSKTLINQITFIAISIGMLLIITVIITSALLVMLISDIYVTQYQQFMILMKALGYSNYKISKYAFGTAIVFSLIMWAISTLATWILITLIIQIITSLGFAIPYGFAFWTLIVSFIIIGISFIGSLIVSSNKIRTQKPASLLTVSNE


Function and Homologs

  • Product: efflux ABC transporter, permease protein
  • Module: putative transport complex
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
  • ABC transporter permease [Mycoplasma mycoides] 3465 100% 0.0 100% WP_023905947.1
  • ABC transporter permease [Mycoplasma mycoides] 3388 100% 0.0 98% WP_017697832.1
  • ABC transporter permease [Mycoplasma mycoides] 3372 100% 0.0 97% WP_039275635.1
  • Equivalent E. coli / JCVI functional protein: EG11657

Expression

  • Expression Level: medium
  • Expression Level Hypothesis: Cells must regularly discard waste products as the cell grows and consumes resources. Although the exact function of this gene is unknown, we can guess that an ABC transporter must be produced at a medium amount so that the "trash" is taken out, but not so much that resources are thrown out unnecessarily.
  • Expression Level References and Description: I was unable to determine which exact ABC transporter this gene encodes, but as a whole, ABC transporters generally have a medium expression rate according to the Mgenitalium Data Set.
  • Expression Time: Late
  • Expression Level Hypothesis: The cell does not need to immediately start disposing of toxins. Waste accumulation and disposal process is a result time and decay, so it follows that this protein should be expressed later on in the cell's lifecycle.
  • Expression Time References and Description: Gene description.

Gene Context

  • Other Components: Unknown
  • Possible Dependencies: Protein Salvage
  • Process: metabolite uptake transport
    • Inputs: ATP, molecule being transported
    • Outputs: ADP, phosphate, molecule being transported
    • Reference: [[4]]

Construct

  • Synthesis Score: The synthesis score of your construct: 1, 2,3
  • Predicted Translation Rate: Prediction of construct translation rate from the RBS calculator
  • Design Notes and Details: For example, had to use a rare codon to fix folding energy;
  • GenBank File: A link to the GenBank file. file