MMSYN1 0687

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Author Information

Maria Gutierrez

Basic Information

  • ID: MMSYN1_0687
  • Name: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit
  • Organism: JCVI-Syn3.0
  • Description: The data suggest that GatB is involved in recognition of the mischarged AA-tRNA. JBC. This gene allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln). BLAST NCBI
  • DNA Length: 1440
  • DNA sequence:

ATG CAA AAC TTC GAA ATC ATT ATT GGT GTC GAA AAC CAC GTC GAG TTG AAG ACG AAC TCA AAA ATG TTC TCC CCT TCC AAG GTA AGC TAC GGA CAA ACG CCT AAC ACA CTT GCC AAC GAA ATT GAC CTT GCA TAT CCT GGC ACG TTA CCA AGC GTC AAT AAG AAG GGC GTA GAA CTG GCG ATT TTA GCC TGT AAC GCA TTG AAC ATG CAA ATC GAT ACA TTG CTG ACG TTC GAT CGT AAA AAT TAT TTC TAT CCA GAC CTT ACT AAG GGG TTC CAA ATT ACG CAG CAG TTT AAC CCT ATC GGA ACC AAT GGA AGC CTT GAG ATT ACT CTG GAA AAC GGA AAC AAA AAG GTC ATC GAA ATT GAG CGT CTG CAC ATC GAA GAA GAC ACG GCT AAA CAA GTA CAC AAA GAC AAC CTG ACG TAC CTG GAC TAC AAT CGT AGT GGG GTG GGG TTA ATT GAA ATC GTG ACC AAG CCC GTT TTG CGC TCT GCG GAG GAG GCT TGT TTA TAC GTC GAG AAG TTG CGT GAG ATT CTT CTG TTT CTT AAC GTG AGC GAC GTG AAG ATG AAT GAG GGA TCT TTG CGT ACG GAT CTT AAC ATC TCG CTG CGT CCG TAC GGT TCC GAC AAG TTC TCG AAC AAA GTC GAG ATC AAA AAT TTG AAC TCC ATT TCT AAC ATC AAA AAA GCG GTA GAA TTT GAG ATC AAC CGT CAG AAA GAA ATT TTG CTT AAA AAT CAG ATC GTC GAA CAA CAA ACG CGC CGC TTT GAC GAC CAG ACG AGT TCG ACT ATT CTT ATG CGT TCG AAG ATT GAC TCC ATC GAT TAC CGT TAT TTT CGC GAA CCT AAC ATT TTC CCG ATT CAA CTT GAC CAG AAA TGG GTC GAC CAA ATT ATC TCA AAT TCT CCT GAA CTG GCC GAT CAG AAA CGT ATT CGT TAT GTT AAT GAG CTG GGC CTG ACT TCG GAG GAT GCA AAC ATT ATT TTG ACG TCC TTG GAG ATG ACT AAC TTC TTC GAA AAA ACT ATC AAG TCT ACT ACG AAC TAT AAT AAG GTC GCA AAA ATG CTG ATT TCG GAG ATC CAA GCT AAA CTT AAT TTA GAA AAT AAG ACT ATT GAC CAA ATC AAG TTA AGC CCT GAG AAC CTG GCG AGC GTT ATC AAT CTG ATT GAC AAG AAT ATT ATC TCA TCG AAG CAG ACT AAA GTT ATT ATG CCA ATT ATC CTT GAC TCT AAT ACG GAA ACA GTT GAA CAG ATC GTC GAG CGT CTT AAC CTG AAG CTT ATT ACA AAC AAG GAC GAA ATT TCG AAG TTA CTT GTC AAT ATC ATC AAT CAA AAC AAA GAG CTT TTG AAT CAG TAC TCA ACA CGT CCC GAA CGC GTT ATC AAG ACG ATC ATG GGC CAG TTG ATG AAG CAA ACA AAT GGG AAC GTA GAC CCC GAA ATC GCA AAC GAA ATT GTG ATC AAG GAA ATT GAG AAA AAT CTG TAA

  • Amino Acid length: 479
  • Amino Acid sequence:

MQNFEIIIGVENHVELKTNSKMFSPSKVSYGQTPNTLANEIDLAYPGTLPSVNKKGVELAILACNALNMQIDTLLTFDRKNYFYPDLTKGFQITQQFNPIGTNGSLEITLENGNKKVIEIERLHIEEDTAKQVHKDNLTYLDYNRSGVGLIEIVTKPVLRSAEEACLYVEKLREILLFLNVSDVKMNEGSLRTDLNISLRPYGSDKFSNKVEIKNLNSISNIKKAVEFEINRQKEILLKNQIVEQQTRRFDDQTSSTILMRSKIDSIDYRYFREPNIFPIQLDQKWVDQIISNSPELADQKRIRYVNELGLTSEDANIILTSLEMTNFFEKTIKSTTNYNKVAKMLISEIQAKLNLENKTIDQIKLSPENLASVINLIDKNIISSKQTKVIMPIILDSNTETVEQIVERLNLKLITNKDEISKLLVNIINQNKELLNQYSTRPERVIKTIMGQLMKQTNGNVDPEIANEIVIKEIEKNL

Function and Homologs

  • Product: Catalyzes the generation of Asn-tRNA or Gln-tRNA.
  • Closest homologous proteins: The top (max three) homologous proteins to this protein, as identified by BLAST searches.
    • aspartyl/glutamyl-tRNA amidotransferase subunit B [Mycoplasma mycoides], 956/100/0.0/100%, WP_020862912.1
    • aspartyl/glutamyl-tRNA amidotransferase subunit B [Mycoplasma mycoides], 949/100%/0.0/99%, WP_011166929.1
    • aspartyl/glutamyl-tRNA amidotransferase subunit B [Mycoplasma mycoides], 949/100%/0.0/99%, P61345.1
  • Equivalent E. coli / JCVI functional protein: There is no amidotranferase present in E. coli. There are other bacterias, such as Chlamydia trachomatis, that do contain an equivalent process and protein, but E. coli in particular does not.

Expression

  • Expression Level: Medium
  • Expression Level Hypothesis: My gene is involved in catalyzing the generation of two tRNAs, and since there are 20 amino acids needed, then there is not much production required for only two of them. But, since amino acids are the building blocks for all proteins, there is still a 'medium' expression level required for the enzyme that aids in the process to build amino acid chains.
  • Expression Level References and Description: To understand what tRNA was I used Wikipedia. But in order to understand what my gene did I looked it up on UniProt, NCBI and a scientific paper in JBC.
  • Expression Time: Early
  • Expression Level Hypothesis: This gene is used in translation, which occurs during interphase (the first stage of the cell cycle) and then throughout the life of the cell. tRNAs are used at the end of initiation and during elongation. Thus, they are used in an early stage of translation, but not right at the beginning of the cell lifecycle.
  • Expression Time References and Description: I looked up translation to understand how the process works and thus where tRNA would come into play.


Gene Context

  • Other Components: gatC (Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C) MMSYN1_0689
  • Possible Dependencies: pth: aminoacyl-tRNA hydrolase The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Involved in lambda inhibition of host protein synthesis. PTH activity may, directly or indirectly, be the target for lambda bar RNA leading to rap cell death.
  • Process: Translation elongation
    • Inputs: Uncharged tRNA, ATP, Specific amino acid
    • Outputs: Charged tRNA, Pyrophospate, Activated amino acid (+charged tRNA), AMP